SMAD2 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti SMAD2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti SMAD2 (**VPA00182**) antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 61 second exposure. Arrow points to SMAD2 (molecular weight 60 kDa)

Filter by Application:

Rabbit anti SMAD2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SMAD2

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00182 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human SMAD2 antibody recognizes SMAD2 also known as mothers against decapentaplegic homolog 2 , MAD homolog 2, mothers against DPP homolog 2, JV18-1 Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2 and hSMAD2.

SMAD2 belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for SMAD2 (provided by RefSeq, May 2012).

Rabbit anti Human SMAD2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SMAD2 detects a band of approximately 60 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Synthetic peptide corresponding to an internal region of human SMAD2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q15796
Entrez Gene: SMAD2
GO Terms: GO:0001707 mesoderm formation; GO:0003690 double-stranded DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005829 cytosol; GO:0035556 intracellular signal transduction; GO:0030618 transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007182 common-partner SMAD protein phosphorylation; GO:0007183 SMAD protein complex assembly; View More GO Terms; GO:0007352 zygotic specification of dorsal/ventral axis; GO:0008134 transcription factor binding; GO:0009952 anterior/posterior pattern formation; GO:0010718 positive regulation of epithelial to mesenchymal transition; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0017015 regulation of transforming growth factor beta receptor signaling pathway; GO:0031053 primary microRNA processing; GO:0031625 ubiquitin protein ligase binding; GO:0032444 activin responsive factor complex; GO:0034713 type I transforming growth factor beta receptor binding; GO:0043193 positive regulation of gene-specific transcription; GO:0045165 cell fate commitment; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048340 paraxial mesoderm morphogenesis; GO:0051098 regulation of binding; GO:0060021 palate development; GO:0070410 co-SMAD binding; GO:0070411 I-SMAD binding; GO:0070412 R-SMAD binding; GO:0070723 response to cholesterol