- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMAD2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-stranded DNA binding
sequence-specific DNA binding transcription factor activity
intracellular signal transduction
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
transforming growth factor beta receptor signaling pathway
common-partner SMAD protein phosphorylation
SMAD protein complex assembly
zygotic specification of dorsal/ventral axis
transcription factor binding
anterior/posterior pattern formation
positive regulation of epithelial to mesenchymal transition
transcription activator activity
regulation of transforming growth factor beta receptor signaling pathway
primary microRNA processing
ubiquitin protein ligase binding
activin responsive factor complex
type I transforming growth factor beta receptor binding
positive regulation of gene-specific transcription
cell fate commitment
negative regulation of transcription, DNA-dependent
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
regulation of binding
response to cholesterol
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMAD2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SMAD2 antibody detects a band of approximately 52 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available
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