Rabbit anti SMAD1 antibody recognizes SMAD1, also known as mothers against decapentaplegic homolog 1 and transforming growth factor-beta-signaling protein 1. SMAD1 is a transcription factor and belongs to the dwarfin/SMAD family and is activated by bone morphogenetic proteins (BMP) to modulate transcription.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMAD1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human SMAD1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sequence-specific DNA binding transcription factor activity
integral to membrane
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
nuclear inner membrane
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
embryonic pattern specification
identical protein binding
protein kinase binding
BMP signaling pathway
primary microRNA processing
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMAD1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SMAD1 antibody detects a band of approximately 52 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available
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