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Smac/DIABLO antibody

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Western Blot Validated
Anti Smac/DIABLO Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Smac/DIABLO antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to Smac (molecular weight 21 kDa)

Rabbit anti Smac/DIABLO

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00087
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human Smac/DIABLO antibody recognizes human second mitochondria-derived activator of caspase (Smac), otherwise known as direct IAP-binding protein with low pI (DIABLO) or diablo homolog, mitochondrial. Smac is expressed in a variety of tissues and has been identified as a negative regulator of X-linked inhibitor of apoptosis (XIAP), a member of the IAP (inhibitors of apoptosis proteins) family which blocks cell death by caspase inhibition.

Rabbit anti Human Smac/DIABLO antibody detects Smac as a single band of 21 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti Smac detects a band of approximately 21 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 225-239 of human Smac.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NR28
Entrez Gene
DIABLO
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0005758 mitochondrial intermembrane space
GO:0008625 induction of apoptosis via death domain receptors
GO:0008635 activation of caspase activity by cytochrome c
GO:0009898 internal side of plasma membrane
GO:0035631 CD40 receptor complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Smac/DIABLO antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies

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