Smac/DIABLO antibody
Rabbit anti Human Smac/DIABLO antibody recognizes human second mitochondria-derived activator of caspase (Smac), otherwise known as direct IAP-binding protein with low pI (DIABLO) or diablo homolog, mitochondrial. Smac is expressed in a variety of tissues and has been identified as a negative regulator of X-linked inhibitor of apoptosis (XIAP), a member of the IAP (inhibitors of apoptosis proteins) family which blocks cell death by caspase inhibition.
Rabbit anti Human Smac/DIABLO antibody detects Smac as a single band of 21 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Rabbit anti Human Smac/DIABLO antibody detects Smac as a single band of 21 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti Smac detects a band of approximately 21 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 225-239 of human Smac.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NR28
- Entrez Gene
- DIABLO
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0005758 mitochondrial intermembrane space
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0008635 activation of caspase activity by cytochrome c
- GO:0009898 internal side of plasma membrane
- GO:0035631 CD40 receptor complex
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Smac/DIABLO antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
Product Specific References
Further Reading
-
Verhagen, A.M. et al. (2001) Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs.
Genome Biol. 2 (7): REVIEWS3009. -
Wu, G. et al. (2000) Structural basis of IAP recognition by Smac/DIABLO.
Nature. 408 (6815): 1008-12.
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