Smac/DIABLO antibody

Rabbit anti Smac/DIABLO

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Smac/DIABLO

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VPA00087
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Rabbit anti Human Smac/DIABLO antibody recognizes human second mitochondria-derived activator of caspase (Smac), otherwise known as direct IAP-binding protein with low pI (DIABLO) or diablo homolog, mitochondrial. Smac is expressed in a variety of tissues and has been identified as a negative regulator of X-linked inhibitor of apoptosis (XIAP), a member of the IAP (inhibitors of apoptosis proteins) family which blocks cell death by caspase inhibition.

Rabbit anti Human Smac/DIABLO antibody detects Smac as a single band of 21 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti Smac detects a band of approximately 21 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Synthetic peptide corresponding to amino acids 225-239 of human Smac.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Further Reading

  1. Verhagen, A.M. et al. (2001) Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs.
    Genome Biol. 2 (7): REVIEWS3009.
  2. Wu, G. et al. (2000) Structural basis of IAP recognition by Smac/DIABLO.
    Nature. 408 (6815): 1008-12.

UniProt
Q9NR28
Entrez Gene
DIABLO
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0005758 mitochondrial intermembrane space
GO:0008625 induction of apoptosis via death domain receptors
GO:0008635 activation of caspase activity by cytochrome c
GO:0009898 internal side of plasma membrane
GO:0035631 CD40 receptor complex
View more products with SMAC/DIABLO specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products