SLP-76 antibody

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Goat anti Human SLP-76 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1121 E WB 0.1 mg
Goat anti Human SLP-76 antibody recognises the SH2 domain-containing leucocyte protein (SLP-76), a 76 kDa tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage.

SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human SLP-76 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence C-ALRNVPFRSEV corresponding to the N-terminus of SLP-76 (NP_005556).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13094 Related reagents
Entrez Gene
LCP2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0006955 immune response
GO:0030168 platelet activation
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of SLP-76 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Western Blotting 0.5 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1121 detects a band of approximately 75kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Application Based External Images

Immunofluorescence

Product Specific References

References for SLP-76 antibody

  1. Lasserre, R. et al. (2011) Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation.
    J Cell Biol. 195 (5): 839-53.
  2. Lasserre, R. et al. (2010) Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.
    EMBO J. 29: 2301-14.