Rabbit anti SKP2 antibody
recognizes S-phase kinase-associated protein 2 (SKP2), also known as F-box protein Skp2, p45skp2 and Cyclin-A/CDK2-associated protein p45. SKP2 is a ubiquitin ligase, a critical component of the SKP2-SCF complex (Nakayama and Nakayama 2005
). It is frequently overexpressed in human cancer (Chiarle et al. 2002
). Loss of SKP2 can promote senescence in response to oncogene activation (Lin et al. 2010
). It plays a role in regulating cancer stem cell populations (Chan et al. 2013
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SKP2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- A synthetic peptide corresponding to amino acids 171-182 of human SKP2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
G1/S transition of mitotic cell cycle
S phase of mitotic cell cycle
SCF ubiquitin ligase complex
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SKP2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SKP2 antibody detects a band of approximately 47 kDa in 293T cell lysate and mouse spleen tissue lysate under reducing conditions
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Secondary Antibodies Available