SIRT1 antibody
Rabbit anti SIRT1 antibody recognizes silent mating type information regulation 2 homolog 1 (SIRT1) also known as NAD-dependent protein deacetylase sirtuin-1 (SIRT1) and regulatory protein SIR2 homolog 1.
SIRT1 is a member of the sirtuin family of deacetylases that play an important role in regulating gene expression (Roth and Chen 2014). Due to its function in DNA repair and tumorigenesis, SIRT1 has been identified as a potential therapeutic target for cancer (Roth and Chen 2014).
SIRT1 is a member of the sirtuin family of deacetylases that play an important role in regulating gene expression (Roth and Chen 2014). Due to its function in DNA repair and tumorigenesis, SIRT1 has been identified as a potential therapeutic target for cancer (Roth and Chen 2014).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to SIRT1 was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to the carboxy terminus of human SIRT1
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q96EB6
- Entrez Gene
- SIRT1
- GO Terms
- GO:0006260 DNA replication
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0000183 chromatin silencing at rDNA
- GO:0002039 p53 binding
- GO:0003714 transcription corepressor activity
- GO:0042393 histone binding
- GO:0006915 apoptosis
- GO:0005637 nuclear inner membrane
- GO:0005737 cytoplasm
- GO:0005677 chromatin silencing complex
- GO:0005719 nuclear euchromatin
- GO:0005720 nuclear heterochromatin
- GO:0005730 nucleolus
- GO:0006281 DNA repair
- GO:0006343 establishment of chromatin silencing
- GO:0006344 maintenance of chromatin silencing
- GO:0006364 rRNA processing
- GO:0006642 triglyceride mobilization
- GO:0007517 muscle organ development
- GO:0007569 cell aging
- GO:0008022 protein C-terminus binding
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0009267 cellular response to starvation
- GO:0042981 regulation of apoptosis
- GO:0016564 transcription repressor activity
- GO:0016575 histone deacetylation
- GO:0016605 PML body
- GO:0018394 peptidyl-lysine acetylation
- GO:0031937 positive regulation of chromatin silencing
- GO:0032071 regulation of endodeoxyribonuclease activity
- GO:0033158 regulation of protein import into nucleus, translocation
- GO:0033553 rDNA heterochromatin
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0034983 peptidyl-lysine deacetylation
- GO:0042127 regulation of cell proliferation
- GO:0043398 HLH domain binding
- GO:0043425 bHLH transcription factor binding
- GO:0043433 negative regulation of transcription factor activity
- GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
- GO:0044419 interspecies interaction between organisms
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045739 positive regulation of DNA repair
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046969 NAD-dependent histone deacetylase activity (H3-K9 specific)
- GO:0050872 white fat cell differentiation
- GO:0051097 negative regulation of helicase activity
- GO:0070403 NAD+ binding
- Regulatory
- For research purposes only
Applications of SIRT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/400 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SIRT1 recognizes bands of approximately 117 kDa when run under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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