SIRT1 antibody

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Rabbit anti SIRT1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2420 WB 0.1 mg
Rabbit anti SIRT1 antibody recognizes silent mating type information regulation 2 homolog 1 (SIRT1) also known as NAD-dependent protein deacetylase sirtuin-1 (SIRT1) and regulatory protein SIR2 homolog 1.

SIRT1 is a member of the sirtuin family of deacetylases that play an important role in regulating gene expression (Roth and Chen 2014). Due to its function in DNA repair and tumorigenesis, SIRT1 has been identified as a potential therapeutic target for cancer (Roth and Chen 2014).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to SIRT1 was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to the carboxy terminus of human SIRT1
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q96EB6 Related reagents
Entrez Gene
SIRT1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0000183 chromatin silencing at rDNA
GO:0002039 p53 binding
GO:0003714 transcription corepressor activity
GO:0042393 histone binding
GO:0006915 apoptosis
GO:0005637 nuclear inner membrane
GO:0005737 cytoplasm
GO:0005677 chromatin silencing complex
GO:0005719 nuclear euchromatin
GO:0005720 nuclear heterochromatin
GO:0005730 nucleolus
GO:0006281 DNA repair
GO:0006343 establishment of chromatin silencing
GO:0006344 maintenance of chromatin silencing
GO:0006364 rRNA processing
GO:0006642 triglyceride mobilization
GO:0007517 muscle organ development
GO:0007569 cell aging
GO:0008022 protein C-terminus binding
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0009267 cellular response to starvation
GO:0042981 regulation of apoptosis
GO:0016564 transcription repressor activity
GO:0016575 histone deacetylation
GO:0016605 PML body
GO:0018394 peptidyl-lysine acetylation
GO:0031937 positive regulation of chromatin silencing
GO:0032071 regulation of endodeoxyribonuclease activity
GO:0033158 regulation of protein import into nucleus, translocation
GO:0033553 rDNA heterochromatin
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0034983 peptidyl-lysine deacetylation
GO:0042127 regulation of cell proliferation
GO:0043398 HLH domain binding
GO:0043425 bHLH transcription factor binding
GO:0043433 negative regulation of transcription factor activity
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0044419 interspecies interaction between organisms
GO:0045599 negative regulation of fat cell differentiation
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0046969 NAD-dependent histone deacetylase activity (H3-K9 specific)
GO:0050872 white fat cell differentiation
GO:0051097 negative regulation of helicase activity
GO:0070403 NAD+ binding
Regulatory
For research purposes only

Applications of SIRT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti SIRT1 recognizes bands of approximately 117 kDa when run under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*