SHC1 (pTyr349) antibody

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Rabbit anti SHC1 (pTyr349)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2749
Datasheet
WB
SDS
50 µl loader

Rabbit anti SHC1 (pTyr349) antibody recognizes SHC-transforming protein 1 when phosphorylated on tyrosine 349. The Shc gene encodes p46Shc, p52Shc and p66Shc, which differ at the N-terminus (Pelicci et al. 1992). Residue tyrosine 349 refers to isoform p66 and tyrosine 239 in p52. This phosphorylation contributes to the interaction of SHC1 with GRB2 (van der Geer et al. 1996).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SHC1 (pTyr349) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 349 of human SHC1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P29353
Entrez Gene
SHC1
Regulatory
For research purposes only

Applications of SHC1 (pTyr349) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
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Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
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