SFRP1 antibody

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Rabbit anti SFRP1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1727 Datasheet	E P* WB SDS	50 µg	Log in		Get Quote

Rabbit anti Human SFRP1 antibody recognizes the human Secreated Frizzled-Related Protein 1 (SFRP1), a multi functional secreted protein belonging to the sFRP family.

sFRPS function as modulators of Wnt signalling and have a role in the regulation of cell growth and differentiation in specific cell types. SFRP1 is widely expressed in human tissues (though absent from the lung, liver and peripheral blood leucocytes). SFRP1 has an anti-proliferative effect upon vascular cells, delaying the G1 phase and entry into S-phase in the cell cycle.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Bovine
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to Human SFRP1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Sodium Azide (NaN₃)

Immunogen

A synthetic peptide corresponding to amino acids 43-54 of human SFRP1.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q8N474
Entrez Gene: SFRP1
GO Terms: GO:0005886 plasma membrane; GO:0004197 cysteine-type endopeptidase activity; GO:0001649 osteoblast differentiation; GO:0001654 eye development; GO:0001657 ureteric bud development; GO:0001843 neural tube closure; GO:0001944 vasculature development; GO:0002244 hemopoietic progenitor cell differentiation; GO:0005829 cytosol; GO:0042813 Wnt receptor activity; GO:0005109 frizzled binding; GO:0005578 proteinaceous extracellular matrix; GO:0005615 extracellular space; GO:0006309 DNA fragmentation involved in apoptotic nuclear change; GO:0007420 brain development; GO:0008144 drug binding; GO:0008201 heparin binding; GO:0061133 endopeptidase activator activity; GO:0008584 male gonad development; GO:0008585 female gonad development; GO:0042802 identical protein binding; GO:0009267 cellular response to starvation; GO:0042493 response to drug; GO:0009950 dorsal/ventral axis specification; GO:0009986 cell surface; GO:0010551 regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010564 regulation of cell cycle process; GO:0010719 negative regulation of epithelial to mesenchymal transition; GO:0014034 neural crest cell fate commitment; GO:0014070 response to organic cyclic compound; GO:0016563 transcription activator activity; GO:0016566 specific transcriptional repressor activity; GO:0017147 Wnt-protein binding; GO:0022601 menstrual cycle phase; GO:0030165 PDZ domain binding; GO:0030279 negative regulation of ossification; GO:0030307 positive regulation of cell growth; GO:0030308 negative regulation of cell growth; GO:0030336 negative regulation of cell migration; GO:0030514 negative regulation of BMP signaling pathway; GO:0032582 negative regulation of gene-specific transcription; GO:0032855 positive regulation of Rac GTPase activity; GO:0033689 negative regulation of osteoblast proliferation; GO:0035019 somatic stem cell maintenance; GO:0043065 positive regulation of apoptosis; GO:0043066 negative regulation of apoptosis; GO:0043193 positive regulation of gene-specific transcription; GO:0043508 negative regulation of JUN kinase activity; GO:0044344 cellular response to fibroblast growth factor stimulus; GO:0045578 negative regulation of B cell differentiation; GO:0045600 positive regulation of fat cell differentiation; GO:0045668 negative regulation of osteoblast differentiation; GO:0045671 negative regulation of osteoclast differentiation; GO:0045765 regulation of angiogenesis; GO:0045880 positive regulation of smoothened signaling pathway; GO:0046676 negative regulation of insulin secretion; GO:0046851 negative regulation of bone remodeling; GO:0048147 negative regulation of fibroblast proliferation; GO:0048546 digestive tract morphogenesis; GO:0050679 positive regulation of epithelial cell proliferation; GO:0050680 negative regulation of epithelial cell proliferation; GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation; GO:0051496 positive regulation of stress fiber assembly; GO:0051894 positive regulation of focal adhesion assembly; GO:0060070 canonical Wnt receptor signaling pathway; GO:0060071 Wnt receptor signaling pathway, planar cell polarity pathway; GO:0060218 hemopoietic stem cell differentiation; GO:0060346 bone trabecula formation; GO:0060527 prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis; GO:0060687 regulation of branching involved in prostate gland morphogenesis; GO:0060766 negative regulation of androgen receptor signaling pathway; GO:0071305 cellular response to vitamin D; GO:0071347 cellular response to interleukin-1; GO:0071356 cellular response to tumor necrosis factor; GO:0071380 cellular response to prostaglandin E stimulus; GO:0071392 cellular response to estradiol stimulus; GO:0071456 cellular response to hypoxia; GO:0071504 cellular response to heparin; GO:0071560 cellular response to transforming growth factor beta stimulus; GO:0071773 cellular response to BMP stimulus; GO:0090246 convergent extension involved in somitogenesis; GO:0090263 positive regulation of canonical Wnt receptor signaling pathway; GO:2000052 positive regulation of non-canonical Wnt receptor signaling pathway; GO:2000054 negative regulation of Wnt receptor signaling pathway involved in dorsal/ventral axis specification; GO:2000080 negative regulation of canonical Wnt receptor signaling pathway involved in controlling type B pancreatic cell proliferation; GO:2000117 negative regulation of cysteine-type endopeptidase activity
Regulatory: For research purposes only

Applications of SFRP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/5000	1/25000
Immunohistology - Paraffin ¹		20ug/ml
Western Blotting	1/200	1/2000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human heart

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
Histar Detection System	STAR3000A	C P	50 Tests	Log in	Get Quote

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