SFPQ antibody

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Rabbit anti SFPQ

Rabbit anti human SFPQ antibody recognizes splicing factor, proline- and glutamine-rich, also known as 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, or regulatory subunit 140. This antibody has been extensively validated for western blotting using whole cell lysates.

SFPQ Antibody with Control Lysate

Rabbit anti human SFPQ antibody recognizes splicing factor, proline- and glutamine-rich, also known as 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, PTB-associated splicing factor, hPOMp100, or polypyrimidine tract-binding protein-associated splicing factor.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00335 WB 100 µl
VPA00335KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SFPQ antibody recognizes SFPQ, also known as 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, hPOMp100, polypyrimidine tract-binding protein-associated splicing factor and splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated).

Rabbit anti Human SFPQ antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SFPQ detects a band of approximately 100 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 676-705 of human SFPQ
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P23246 Related reagents
Entrez Gene
SFPQ Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000380 alternative nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0006281 DNA repair
GO:0006310 DNA recombination
GO:0006350 transcription
GO:0016363 nuclear matrix
GO:0042382 paraspeckles
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SFPQ antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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