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SEC61B antibody

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Anti SEC61B Antibody gallery image 1
Western blot analysis of various cell and tissue lysates using Rabbit anti SEC61B antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking

Rabbit anti SEC61B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2721
Datasheet
WB
SDS
50 µl loader

Rabbit anti SEC61B antibody recognizes protein transport protein Sec61 subunit beta (SEC61B). SEC61B is a subunit of the SEC61 protein translocation channel at the endoplasmic reticulum (Pfeffer et al. 2015). The SEC61 translocon is important for protein import and export (Rapoport 2007). SEC61B is important in EGF R trafficking to the nucleus (Liao and Carpenter 2007). Measurement of SEC61B autoantibody levels may provide an alternative detection indicator for human colorectal cancer (Fan et al. 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SEC61B was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P60468
Entrez Gene
SEC61B
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0016021 integral to membrane
GO:0030433 ER-associated protein catabolic process
GO:0030970 retrograde protein transport, ER to cytosol
GO:0031205 endoplasmic reticulum Sec complex
GO:0048408 epidermal growth factor binding
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of SEC61B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti SEC61B antibody detects a band of approximately 12 kDa in various cell and tissue lysates under reducing conditions
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Request a different product with the SEC61B specificity