SEC61B antibody
Rabbit anti SEC61B antibody recognizes protein transport protein Sec61 subunit beta (SEC61B). SEC61B is a subunit of the SEC61 protein translocation channel at the endoplasmic reticulum (Pfeffer et al. 2015). The SEC61 translocon is important for protein import and export (Rapoport 2007). SEC61B is important in EGF R trafficking to the nucleus (Liao and Carpenter 2007). Measurement of SEC61B autoantibody levels may provide an alternative detection indicator for human colorectal cancer (Fan et al. 2011).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SEC61B was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P60468
- Entrez Gene
- SEC61B
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0016021 integral to membrane
- GO:0030433 ER-associated protein catabolic process
- GO:0030970 retrograde protein transport, ER to cytosol
- GO:0031205 endoplasmic reticulum Sec complex
- GO:0048408 epidermal growth factor binding
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of SEC61B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SEC61B antibody detects a band of approximately 12 kDa in various cell and tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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