SART3 antibody

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Anti SART3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human SART3 antibody (**VPA00142**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to SART3 (molecular weight 140 kDa).

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Goat anti SART3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SART3

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VPA00142 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human SART3 antibody recognizes the squamous cell carcinoma antigen recognized by T-cells 3 (SART3), also known as hSART-3 or Tip110.

SART3 is an RNA-binding nuclear protein and tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. SART3 is an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle and is thought to be involved in the regulation of mRNA splicing (provided by RefSeq, Jul 2008).

Goat anti Human SART3 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SART3 detects a band of approximately 140 kDa in MCF7 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-MSNADFAKLFLRK, from the C Terminus of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch..
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: Q15020
Entrez Gene: SART3
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0003723 RNA binding; GO:0005737 cytoplasm; GO:0006396 RNA processing; GO:0016607 nuclear speck