RXR Alpha antibody

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Anti RXR Alpha Antibody thumbnail image 2

Anti RXR Alpha Antibody thumbnail image 3

Anti RXR Alpha Antibody gallery image 1 — Western blot analysis of HeLa cell, nuclear lysate (A) + pre-incubation with immunizing peptide (B). K562 cell, nuclear lysate (C) + immunizing peptide (D). 35μg protein in RIPA buffer for each lane. Detection is by chemiluminescence.

Anti RXR Alpha Antibody gallery image 2 — Immunofluorescence image of paraformaldehyde fixed, triton permeablized MCF7 epitheloid cells stained with Goat anti RXR alpha antibody (**AHP1116**) for 1 hr at 10μgml^-1 followed by Alexa Fluor 488 secondary (green). Actin filaments are stained with phalloidin (red) and nuclei are counterstained with DAPI (blue). Unimmunized goat IgG is used as a negative control.

Anti RXR Alpha Antibody gallery image 3 — Flow cytometry profile of paraformaldehyde fixed, triton permealized MCF7 breast cancer derived, epitheloid cells labeled with Goat anti RXR alpha antibody (**AHP1116**) for 1 hr at a concentration of 10μgml^-1 followed by Alexa Fluor 488 secondary. Unimmunized goat IgG (balck line) is used as a negative control.

Goat anti RXR Alpha

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1116 Datasheet	E WB SDS	0.1 mg	Log in		Get Quote

Goat anti Human RXR alpha antibody detects an epitope within the internal region of retinoid X receptor alpha (RXR alpha). RXR alpha is a ~54 kDa member of the steroid/ thyroid receptor superfamily of transcriptional regulators, and is a nuclear receptor that mediates the biological effects of retinoids through its involvement in retinoic acid-mediated gene activation. RXR alpha regulates gene transcription by recruiting coactivators or corepressors to their promoter sequences.

RXRs play important roles in multiple signalling pathways, including cell growth, differentiation, homeostasis, metabolism, and development.

Goat anti Human RXR alpha antibody does not cross-react with either RXR beta or gamma.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human RXR alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide
0.5%	Bovine Serum Albumin

Immunogen

Peptide sequence CQVNSSLTSPTGRGSM corresponding to the internal region of RXR alpha (NP_002948).

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P19793
Entrez Gene: RXRA
GO Terms: GO:0000790 nuclear chromatin; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003706 ligand-regulated transcription factor activity; GO:0003707 steroid hormone receptor activity; GO:0003713 transcription coactivator activity; GO:0004886 retinoid-X receptor activity; GO:0005654 nucleoplasm; GO:0006766 vitamin metabolic process; GO:0008203 cholesterol metabolic process; GO:0008270 zinc ion binding; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0019899 enzyme binding; GO:0032526 response to retinoic acid; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0042809 vitamin D receptor binding; GO:0044255 cellular lipid metabolic process; GO:0044323 retinoic acid-responsive element binding; GO:0044419 interspecies interaction between organisms; GO:0046982 protein heterodimerization activity
Regulatory: For research purposes only

Applications of RXR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/15000	1/20000
Western Blotting		0.5	3ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1116 detects a band of approximately 55kDa in human epidermoid carcinoma A431 lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Application Based External Images

Immunofluorescence

Immunoprecipitation

Product Specific References

References for RXR Alpha antibody

Pestka, A. et al. (2011) Retinoid X receptor α and retinoids are key regulators in apoptosis of trophoblasts of patients with recurrent miscarriages.
J Mol Endocrinol. 47 (2): 145-56.
Zhang, Z. et al. (2010) Constitutive Activation of the Mitogen Activated Protein Kinase Pathway Impairs Vitamin D Signaling in Human Prostate Epithelial Cells
J Cell Physiol. 224: 433-42.

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