RXR Alpha antibody
Goat anti Human RXR alpha antibody detects an epitope within the internal region of retinoid X receptor alpha (RXR alpha). RXR alpha is a ~54 kDa member of the steroid/ thyroid receptor superfamily of transcriptional regulators, and is a nuclear receptor that mediates the biological effects of retinoids through its involvement in retinoic acid-mediated gene activation. RXR alpha regulates gene transcription by recruiting coactivators or corepressors to their promoter sequences.
RXRs play important roles in multiple signalling pathways, including cell growth, differentiation, homeostasis, metabolism, and development.
Goat anti Human RXR alpha antibody does not cross-react with either RXR beta or gamma.
RXRs play important roles in multiple signalling pathways, including cell growth, differentiation, homeostasis, metabolism, and development.
Goat anti Human RXR alpha antibody does not cross-react with either RXR beta or gamma.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human RXR alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence CQVNSSLTSPTGRGSM corresponding to the internal region of RXR alpha (NP_002948).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19793
- Entrez Gene
- RXRA
- GO Terms
- GO:0000790 nuclear chromatin
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003706 ligand-regulated transcription factor activity
- GO:0003707 steroid hormone receptor activity
- GO:0003713 transcription coactivator activity
- GO:0004886 retinoid-X receptor activity
- GO:0005654 nucleoplasm
- GO:0006766 vitamin metabolic process
- GO:0008203 cholesterol metabolic process
- GO:0008270 zinc ion binding
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0019899 enzyme binding
- GO:0032526 response to retinoic acid
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0042809 vitamin D receptor binding
- GO:0044255 cellular lipid metabolic process
- GO:0044323 retinoic acid-responsive element binding
- GO:0044419 interspecies interaction between organisms
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of RXR Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/15000 | 1/20000 | |
| Western Blotting | 0.5 | 3ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1116 detects a band of approximately 55kDa in human epidermoid carcinoma A431 lysates. A minimum incubation time of 1 hour is recommended with this antibody.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for RXR Alpha antibody
-
Pestka, A. et al. (2011) Retinoid X receptor α and retinoids are key regulators in apoptosis of trophoblasts of patients with recurrent miscarriages.
J Mol Endocrinol. 47 (2): 145-56. -
Zhang, Z. et al. (2010) Constitutive Activation of the Mitogen Activated Protein Kinase Pathway Impairs Vitamin D Signaling in Human Prostate Epithelial Cells
J Cell Physiol. 224: 433-42.
Further Reading
-
Lee, H.Y. et al. (2000) Stress pathway activation induces phosphorylation of retinoid X receptor.
J Biol Chem. 275 (41): 32193-9. -
Ghosh, J.C. et al. (2002) Interactions that determine the assembly of a retinoid X receptor/corepressor complex.
Proc Natl Acad Sci U S A. 99 (9): 5842-7. -
Konta, T. et al. (2001) Selective roles of retinoic acid receptor and retinoid x receptor in the suppression of apoptosis by all-trans-retinoic acid.
J Biol Chem. 276 (16): 12697-701.
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