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RXR Alpha antibody

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Anti RXR Alpha Antibody thumbnail image 1 Anti RXR Alpha Antibody thumbnail image 2 Anti RXR Alpha Antibody thumbnail image 3
Anti RXR Alpha Antibody gallery image 1
Western blot analysis of HeLa cell, nuclear lysate (A) + pre-incubation with immunizing peptide (B). K562 cell, nuclear lysate (C) + immunizing peptide (D). 35μg protein in RIPA buffer for each lane. Detection is by chemiluminescence.
Anti RXR Alpha Antibody gallery image 2
Immunofluorescence image of paraformaldehyde fixed, triton permeablized MCF7 epitheloid cells stained with Goat anti RXR alpha antibody (AHP1116) for 1 hr at 10μgml-1 followed by Alexa Fluor 488 secondary (green). Actin filaments are stained with phalloidin (red) and nuclei are counterstained with DAPI (blue). Unimmunized goat IgG is used as a negative control.
Anti RXR Alpha Antibody gallery image 3
Flow cytometry profile of paraformaldehyde fixed, triton permealized MCF7 breast cancer derived, epitheloid cells labeled with Goat anti RXR alpha antibody (AHP1116) for 1 hr at a concentration of 10μgml-1 followed by Alexa Fluor 488 secondary. Unimmunized goat IgG (balck line) is used as a negative control.

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WB F

Goat anti RXR Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RXR Alpha

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
AHP1116
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human RXR alpha antibody detects an epitope within the internal region of retinoid X receptor alpha (RXR alpha). RXR alpha is a ~54 kDa member of the steroid/ thyroid receptor superfamily of transcriptional regulators, and is a nuclear receptor that mediates the biological effects of retinoids through its involvement in retinoic acid-mediated gene activation. RXR alpha regulates gene transcription by recruiting coactivators or corepressors to their promoter sequences.

RXRs play important roles in multiple signalling pathways, including cell growth, differentiation, homeostasis, metabolism, and development.

Goat anti Human RXR alpha antibody does not cross-react with either RXR beta or gamma.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human RXR alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence CQVNSSLTSPTGRGSM corresponding to the internal region of RXR alpha (NP_002948).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/15000 1/20000
Western Blotting 0.5 3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1116 detects a band of approximately 55kDa in human epidermoid carcinoma A431 lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for RXR Alpha antibody

  1. Pestka, A. et al. (2011) Retinoid X receptor α and retinoids are key regulators in apoptosis of trophoblasts of patients with recurrent miscarriages.
    J Mol Endocrinol. 47 (2): 145-56.
  2. Zhang, Z. et al. (2010) Constitutive Activation of the Mitogen Activated Protein Kinase Pathway Impairs Vitamin D Signaling in Human Prostate Epithelial Cells
    J Cell Physiol. 224: 433-42.

Further Reading

  1. Lee, H.Y. et al. (2000) Stress pathway activation induces phosphorylation of retinoid X receptor.
    J Biol Chem. 275 (41): 32193-9.
  2. Ghosh, J.C. et al. (2002) Interactions that determine the assembly of a retinoid X receptor/corepressor complex.
    Proc Natl Acad Sci U S A. 99 (9): 5842-7.
  3. Konta, T. et al. (2001) Selective roles of retinoic acid receptor and retinoid x receptor in the suppression of apoptosis by all-trans-retinoic acid.
    J Biol Chem. 276 (16): 12697-701.

Immunofluorescence

Immunoprecipitation

Synonyms
Retinoid Acid Receptor Alpha
RRID
AB_808734
UniProt
P19793
Entrez Gene
RXRA
GO Terms
GO:0000790 nuclear chromatin
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003706 ligand-regulated transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0003713 transcription coactivator activity
GO:0004886 retinoid-X receptor activity
GO:0005654 nucleoplasm
GO:0006766 vitamin metabolic process
GO:0008203 cholesterol metabolic process
GO:0008270 zinc ion binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0019899 enzyme binding
GO:0032526 response to retinoic acid
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0042809 vitamin D receptor binding
GO:0044255 cellular lipid metabolic process
GO:0044323 retinoic acid-responsive element binding
GO:0044419 interspecies interaction between organisms
GO:0046982 protein heterodimerization activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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