RPS3 antibody
Rabbit anti RPS3
Rabbit anti human RPS3 antibody recognizes 40S ribosomal protein S3, also known as IMR-90 ribosomal protein S3. This antibody has been extensively validated for western blotting using whole cell lysates.
RPS3 Antibody with Control Lysate
Rabbit anti human RPS3 antibody recognizes 40S ribosomal protein S3, also known as IMR-90 ribosomal protein S3. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00367 | WB | 100 µl | ||||
| VPA00367KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RPS3 antibody recognizes RPS3, also known as 40S ribosomal protein S3 and IMR-90 ribosomal protein S3.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPS3 gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extra-ribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of RPS3 in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. RPS3 is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS3 dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for RPS3 (provided by RefSeq, May 2012).
Rabbit anti Human RPS3 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPS3 gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extra-ribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of RPS3 in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. RPS3 is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS3 dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for RPS3 (provided by RefSeq, May 2012).
Rabbit anti Human RPS3 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RPS3 detects a band of approximately 27 kDa in Jurkat cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the C-terminal region of human RPS3
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P23396 Related reagents
- Entrez Gene
- RPS3 Related reagents
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0003684 damaged DNA binding
- GO:0003729 mRNA binding
- GO:0003735 structural constituent of ribosome
- GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
- GO:0004519 endonuclease activity
- GO:0005634 nucleus
- GO:0006413 translational initiation
- GO:0006414 translational elongation
- GO:0006415 translational termination
- GO:0006917 induction of apoptosis
- GO:0006919 activation of caspase activity
- GO:0006974 response to DNA damage stimulus
- GO:0019083 viral transcription
- GO:0019901 protein kinase binding
- GO:0031018 endocrine pancreas development
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032587 ruffle membrane
- GO:0045738 negative regulation of DNA repair
- GO:0051059 NF-kappaB binding
- GO:0051536 iron-sulfur cluster binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RPS3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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