RPS3 antibody

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Rabbit anti RPS3

Rabbit anti human RPS3 antibody recognizes 40S ribosomal protein S3, also known as IMR-90 ribosomal protein S3. This antibody has been extensively validated for western blotting using whole cell lysates.

RPS3 Antibody with Control Lysate

Rabbit anti human RPS3 antibody recognizes 40S ribosomal protein S3, also known as IMR-90 ribosomal protein S3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00367 WB 100 µl
VPA00367KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RPS3 antibody recognizes RPS3, also known as 40S ribosomal protein S3 and IMR-90 ribosomal protein S3.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPS3 gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extra-ribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of RPS3 in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. RPS3 is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS3 dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for RPS3 (provided by RefSeq, May 2012).

Rabbit anti Human RPS3 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the C-terminal region of human RPS3

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P23396 Related reagents
Entrez Gene
RPS3 Related reagents
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0003684 damaged DNA binding
GO:0003729 mRNA binding
GO:0003735 structural constituent of ribosome
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0004519 endonuclease activity
GO:0005634 nucleus
GO:0006413 translational initiation
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0006974 response to DNA damage stimulus
GO:0019083 viral transcription
GO:0019901 protein kinase binding
GO:0031018 endocrine pancreas development
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032587 ruffle membrane
GO:0045738 negative regulation of DNA repair
GO:0051059 NF-kappaB binding
GO:0051536 iron-sulfur cluster binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPS3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti RPS3 detects a band of approximately 27 kDa in Jurkat cell lysate
Western Blotting
Anti RPS3 detects a band of approximately 27 kDa in Jurkat cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB