RPS14 antibody

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Rabbit anti RPS14

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00246
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human RPS14 antibody recognizes 40S ribosomal protein S14, also known as emetine resistant protein.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Encoded by the RPS14 gene, 40S ribosomal protein S14 a ribosomal protein that is a component of the 40S subunit. This protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS14 dispersed through the genome. In Chinese hamster ovary cells, mutations in RPS14 can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for RPS14 (provided by RefSeq, Jul 2008).

Rabbit anti Human RPS14 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPS14 detects a band of approximately 16 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 118-147 amino acids from the C-terminal region of human RPS14

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P62263
Entrez Gene
RPS14
GO Terms
GO:0000028 ribosomal small subunit assembly
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0022627 cytosolic small ribosomal subunit
GO:0005515 protein binding
GO:0003735 structural constituent of ribosome
GO:0005730 nucleolus
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0019083 viral transcription
GO:0030218 erythrocyte differentiation
GO:0030490 maturation of SSU-rRNA
GO:0031018 endocrine pancreas development
GO:0045182 translation regulator activity
GO:0048027 mRNA 5'-UTR binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RPS14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml