Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Encoded by the RPL7 gene, 60S ribosomal protein L7 is a ribosomal protein that is a component of the 60S subunit. This protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL7 dispersed through the genome (provided by RefSeq, Jul 2008).
Rabbit anti RPL7 antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti RPL7 detects a band of approximately 29 kDa in Raji cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- KLH conjugated synthetic peptide between 6-35 amino acids from the N-terminal region of human RPL7
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0022625 cytosolic large ribosomal subunit
- GO:0003677 DNA binding
- GO:0003729 mRNA binding
- GO:0003735 structural constituent of ribosome
- GO:0006364 rRNA processing
- GO:0006414 translational elongation
- GO:0006415 translational termination
- GO:0019083 viral transcription
- GO:0030528 transcription regulator activity
- GO:0031018 endocrine pancreas development
- GO:0042273 ribosomal large subunit biogenesis
- GO:0042803 protein homodimerization activity
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of RPL7 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|