RPL17 antibody

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Rabbit anti RPL17

Rabbit anti human RPL17 antibody recognizes HCG24487, isoform CRA_c, also known as 60S ribosomal protein L17, 60S ribosomal protein L23 or gene encoding putative NFkB activating protein. This antibody has been extensively validated for western blotting using whole cell lysates.

RPL17 Antibody with Control Lysate

Rabbit anti human RPL17 antibody recognizes HCG24487, isoform CRA_c, also known as 60S ribosomal protein L17, 60S ribosomal protein L23 or gene encoding putative NFkB activating protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00363 WB 100 µl
VPA00363KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RPL17 antibody recognizes RPL17, also known as 60S ribosomal protein L17, 60S ribosomal protein L23 or gene encoding putative NFkB activating protein.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. RPL17 has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL17 dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between RPL17 and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene (provided by RefSeq, Dec 2010).

Rabbit anti Human RPL17 antibody detects bands of 22 and 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 156-184 of human RPL17

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P18621 Related reagents
Entrez Gene
RPL17 Related reagents
GO Terms
GO:0003735 structural constituent of ribosome
GO:0005829 cytosol
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0015934 large ribosomal subunit
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPL17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti RPL17 detects a bands of approximately 22 and 24 kDa in HeLa cell lysate
Western Blotting
Anti RPL17 detects bands of approximately 22 kDa and 24 kDa in HeLa cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB