RPL13A antibody

Rabbit anti RPL13A

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
RPL13A

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VPA00752
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Rabbit anti Human RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13A encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. RPL13A is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL13A dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for RPL13A (provided by RefSeq, Jul 2012).

Rabbit anti Human RPL13A antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RPL13A antibody recognizes a band of approximately 23 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Immunogen
Recombinant protein encompassing part of the center region of human RPL13A
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P40429
Entrez Gene
RPL13A
GO Terms
GO:0003735 structural constituent of ribosome
GO:0005829 cytosol
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0015934 large ribosomal subunit
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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