RPL13A antibody
Rabbit anti RPL13A
RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.
RPL13A Antibody with Control Lysate
RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00752 | WB | 100 µl | ||||
| VPA00752KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13A encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. RPL13A is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL13A dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for RPL13A (provided by RefSeq, Jul 2012).
Rabbit anti Human RPL13A antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13A encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. RPL13A is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL13A dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for RPL13A (provided by RefSeq, Jul 2012).
Rabbit anti Human RPL13A antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RPL13A antibody recognizes a band of approximately 23 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- TRIS buffered glycine
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- 0.01% Thiomersal
<10% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
<10% Glycerol - Immunogen
- Recombinant protein encompassing part of the center region of human RPL13A
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P40429 Related reagents
- Entrez Gene
- RPL13A Related reagents
- GO Terms
- GO:0003735 structural constituent of ribosome
- GO:0005829 cytosol
- GO:0006414 translational elongation
- GO:0006415 translational termination
- GO:0015934 large ribosomal subunit
- GO:0019083 viral transcription
- GO:0031018 endocrine pancreas development
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RPL13A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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