RPL13A antibody

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Rabbit anti RPL13A

RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.

RPL13A Antibody with Control Lysate

RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00752 WB 100 µl
VPA00752KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RPL13A antibody recognizes the 60S ribosomal protein L13a, also known as 23 kDa highly basic protein or tissue specific transplantation antigen 1.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13A encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. RPL13A is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL13A dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for RPL13A (provided by RefSeq, Jul 2012).

Rabbit anti Human RPL13A antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPL13A antibody recognizes a band of approximately 23 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
TRIS buffered glycine
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Immunogen
Recombinant protein encompassing part of the center region of human RPL13A
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P40429 Related reagents
Entrez Gene
RPL13A Related reagents
GO Terms
GO:0003735 structural constituent of ribosome
GO:0005829 cytosol
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0015934 large ribosomal subunit
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RPL13A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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