RPAP3 antibody
Rabbit anti RPAP3 antibody recognizes RNA polymerase II-associated protein 3 (RPAP3), also known as HSpagh and Tah1.
It is a component of the R2TP/PFDL complex (Cloutier et al. 2009) and provides a flexible scaffold for allowing the abundant molecular chaperone HSP90 to interact with other proteins including RNA Pol II, TOR and SMG1, allowing the assembly of important cellular complexes (Martino et al. 2018). It has been suggested that RPAP3 can modulate UV-induced DNA damage (Ni et al. 2009). RPAP3 appears to bind to NEMO, inhibiting the NF-kappaB signaling pathway and enhancing cytotoxicity of the chemotherapeutic agent doxorubicin (Shimada et al. 2011).
It is a component of the R2TP/PFDL complex (Cloutier et al. 2009) and provides a flexible scaffold for allowing the abundant molecular chaperone HSP90 to interact with other proteins including RNA Pol II, TOR and SMG1, allowing the assembly of important cellular complexes (Martino et al. 2018). It has been suggested that RPAP3 can modulate UV-induced DNA damage (Ni et al. 2009). RPAP3 appears to bind to NEMO, inhibiting the NF-kappaB signaling pathway and enhancing cytotoxicity of the chemotherapeutic agent doxorubicin (Shimada et al. 2011).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to RPAP3 was raised by repeated immunization of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547)
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9H6T3
- Entrez Gene
- RPAP3
- GO Terms
- GO:0005488 binding
- Regulatory
- For research purposes only
Applications of RPAP3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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