RPAP3 antibody

100% Secure


Rabbit anti RPAP3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2999
Datasheet
WB
SDS
50 µl loader

Rabbit anti RPAP3 antibody recognizes RNA polymerase II-associated protein 3 (RPAP3), also known as HSpagh and Tah1.

It is a component of the R2TP/PFDL complex (Cloutier et al. 2009) and provides a flexible scaffold for allowing the abundant molecular chaperone HSP90 to interact with other proteins including RNA Pol II, TOR and SMG1, allowing the assembly of important cellular complexes (Martino et al. 2018). It has been suggested that RPAP3 can modulate UV-induced DNA damage (Ni et al. 2009). RPAP3 appears to bind to NEMO, inhibiting the NF-kappaB signaling pathway and enhancing cytotoxicity of the chemotherapeutic agent doxorubicin (Shimada et al. 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to RPAP3 was raised by repeated immunization of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547)

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9H6T3
Entrez Gene
RPAP3
GO Terms
GO:0005488 binding
Regulatory
For research purposes only

Applications of RPAP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg