Rna Helicase A antibody

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Rabbit anti Rna Helicase A

Rabbit anti human RNA helicase A antibody recognizes ATP-dependent RNA helicase A, also known as DHX9, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, RNA helicase A, leukophysin or nuclear DNA helicase II. This antibody has been validated for western blotting using whole cell lysates.

Rna Helicase A Antibody with Control Lysate

Rabbit anti human RNA helicase A antibody recognizes ATP-dependent RNA helicase A, also known as DHX9, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DEAH box protein 9, RNA helicase A, leukophysin or nuclear DNA helicase II.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00298 WB 100 µl
VPA00298KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RNA helicase A antibody recognizes RNA helicase A, also known as DHX9, DDX9, ATP-dependent RNA helicase A, DEAD/H box polypeptide 9, DEAH box protein 9, leukophysin and nuclear DNA helicase II.

The DHX9 gene encodes an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of RNA Helicase A are found on chromosomes 11 and 13 (provided by RefSeq, Feb 2010).

Rabbit anti Human RNA helicase A antibody detects a band of 148 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RNA helicase A detects a band of approximately 148 kDa in Jurkat cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 1-30 of human RNA helicase A
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q08211 Related reagents
Entrez Gene
DHX9 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003725 double-stranded RNA binding
GO:0030529 ribonucleoprotein complex
GO:0004003 ATP-dependent DNA helicase activity
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0070934 CRD-mediated mRNA stabilization
GO:0070937 CRD-mediated mRNA stability complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Rna Helicase A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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