Rabbit anti Human RIP2 antibody recognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.
RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.
Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human RIP2 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- A peptide corresponding to amino acids near the amino terminus of human RIP2.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
protein serine/threonine kinase activity
signal transducer activity
Toll signaling pathway
LIM domain binding
positive regulation of protein ubiquitination
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB cascade
innate immune response
nerve growth factor receptor signaling pathway
CARD domain binding
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of RIP2 antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Western Blotting
- Rabbit anti Human RIP2 detects a band of approximately 60kDa in A431 cell lysates.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Inohara N., et al (1998) RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis.
J Biol Chem. 273:12296-300.
McCarthy J.V., et al (1998) RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase.
J Biol Chem. 273:16968-75.
Thome M., et al (1998) Identification of CARDIAK, a RIP-like kinase that associates with caspase-1.
Curr Biol. 8:885-8.
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