RIP2 antibody

Rabbit anti RIP2 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RIP2
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1590
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SDS Safety Datasheet SDS
C WB 0.1 mg loader
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Rabbit anti Human RIP2 antibody recognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.

RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.

Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human RIP2 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the amino terminus of human RIP2.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Placenta
Western Blotting
Rabbit anti Human RIP2 detects a band of approximately 60kDa in A431 cell lysates.

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Further Reading

  1. Inohara N., et al (1998) RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis.
    J Biol Chem. 273:12296-300.
  2. McCarthy J.V., et al (1998) RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase.
    J Biol Chem. 273:16968-75.
  3. Thome M., et al (1998) Identification of CARDIAK, a RIP-like kinase that associates with caspase-1.
    Curr Biol. 8:885-8.

Synonyms
RICK
RRID
AB_2178253
UniProt
O43353
Entrez Gene
RIPK2
GO Terms
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0030274 LIM domain binding
GO:0031398 positive regulation of protein ubiquitination
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050700 CARD domain binding
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051403 stress-activated MAPK cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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