RIP2 antibody

100% Secure

Anti RIP2 (N-Terminal) Antibody thumbnail image 1

Anti RIP2 (N-Terminal) Antibody thumbnail image 2

Anti RIP2 (N-Terminal) Antibody gallery image 1 — Western blot analysis of whole cell lysates from A431 (A) and K562 (K) cells probed with Rabbit anti RIP-2 (**AHP1590**).

Anti RIP2 (N-Terminal) Antibody gallery image 2 — Immunocytochemical staining of A431 cells with Rabbit anti RIP-2 (**AHP1590**).

Rabbit anti RIP2 (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1590	C WB			0.1 mg

Rabbit anti Human RIP2 antibody recognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.

RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.

Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human RIP2 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)

Immunogen

A peptide corresponding to amino acids near the amino terminus of human RIP2.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: O43353 Related reagents
Entrez Gene: RIPK2 Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0004871 signal transducer activity; GO:0005524 ATP binding; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0007254 JNK cascade; GO:0008063 Toll signaling pathway; GO:0030274 LIM domain binding; GO:0031398 positive regulation of protein ubiquitination; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050700 CARD domain binding; GO:0050852 T cell receptor signaling pathway; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051403 stress-activated MAPK cascade
Regulatory: For research purposes only

Applications of RIP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen			10ug/ml
Western Blotting		0.5	1.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Placenta
Western Blotting: Rabbit anti Human RIP2 detects a band of approximately 60kDa in A431 cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*