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RIP2 antibody

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Anti RIP2 (N-Terminal) Antibody thumbnail image 1 Anti RIP2 (N-Terminal) Antibody thumbnail image 2
Anti RIP2 (N-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysates from A431 (A) and K562 (K) cells probed with Rabbit anti RIP-2 (AHP1590)
Anti RIP2 (N-Terminal) Antibody gallery image 2
Immunocytochemical staining of A431 cells with Rabbit anti RIP-2 (AHP1590)

Rabbit anti RIP2 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1590 C WB 0.1 mg
Rabbit anti Human RIP2 antibody recognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.

RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.

Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human RIP2 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the amino terminus of human RIP2.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O43353 Related reagents
Entrez Gene
RIPK2 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0030274 LIM domain binding
GO:0031398 positive regulation of protein ubiquitination
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050700 CARD domain binding
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of RIP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Placenta
Western Blotting
Rabbit anti Human RIP2 detects a band of approximately 60kDa in A431 cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Inohara N., et al (1998) RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis.
    J Biol Chem. 273:12296-300.
  2. McCarthy J.V., et al (1998) RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase.
    J Biol Chem. 273:16968-75.
  3. Thome M., et al (1998) Identification of CARDIAK, a RIP-like kinase that associates with caspase-1.
    Curr Biol. 8:885-8.
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