RFX1 antibody

Rabbit anti RFX1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
RFX1

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VPA00293
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Rabbit anti Human RFX1 antibody recognizes RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or simply transcription factor RFX1.

The RFX1 gene is a member of the regulatory factor X family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by the RFX1 gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes (provided by RefSeq, Jul 2008).

Rabbit anti Human RFX1 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RFX1 detects a band of approximately 140 kDa in Jurkat cell lysate.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 896-925 of human RFX1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P22670
Entrez Gene
RFX1
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0006355 regulation of transcription, DNA-dependent
GO:0006955 immune response
GO:0030528 transcription regulator activity
View more products with RFX1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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