RFX1 antibody

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Anti RFX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RFX1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 115 second exposure. Arrow points to RFX1 (molecular weight 140 kDa)

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Rabbit anti RFX1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: RFX1

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VPA00293 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human RFX1 antibody recognizes RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or simply transcription factor RFX1.

The RFX1 gene is a member of the regulatory factor X family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by the RFX1 gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes (provided by RefSeq, Jul 2008).

Rabbit anti Human RFX1 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RFX1 detects a band of approximately 140 kDa in Jurkat cell lysate.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 896-925 of human RFX1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P22670
Entrez Gene: RFX1
GO Terms: GO:0005515 protein binding; GO:0003677 DNA binding; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0005634 nucleus; GO:0006355 regulation of transcription, DNA-dependent; GO:0006955 immune response; GO:0030528 transcription regulator activity