RFX1 antibody

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Rabbit anti RFX1

Rabbit anti human RFX1 antibody recognizes the MHC class II regulatory factor RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or transcription factor RFX1. This antibody has been extensively validated for western blotting using whole cell lysates.

RFX1 Antibody with Control Lysate

Rabbit anti human RFX1 antibody recognizes the MHC class II regulatory factor RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or transcription factor RFX1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00293 WB 100 µl
VPA00293KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RFX1 antibody recognizes RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or simply transcription factor RFX1.

The RFX1 gene is a member of the regulatory factor X family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by the RFX1 gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes (provided by RefSeq, Jul 2008).

Rabbit anti Human RFX1 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 896-925 of human RFX1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P22670 Related reagents
Entrez Gene
RFX1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0006355 regulation of transcription, DNA-dependent
GO:0006955 immune response
GO:0030528 transcription regulator activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RFX1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti RFX1 detects a band of approximately 140 kDa in Jurkat cell lysate
Western Blotting
Anti RFX1 detects a band of approximately 140 kDa in Jurkat cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB