RFX1 antibody
Rabbit anti RFX1
Rabbit anti human RFX1 antibody recognizes the MHC class II regulatory factor RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or transcription factor RFX1. This antibody has been extensively validated for western blotting using whole cell lysates.
RFX1 Antibody with Control Lysate
Rabbit anti human RFX1 antibody recognizes the MHC class II regulatory factor RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or transcription factor RFX1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00293 | WB | 100 µl | ||||
| VPA00293KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RFX1 antibody recognizes RFX1, also known as enhancer factor C, regulatory factor X1, trans-acting regulatory factor 1 or simply transcription factor RFX1.
The RFX1 gene is a member of the regulatory factor X family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by the RFX1 gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes (provided by RefSeq, Jul 2008).
Rabbit anti Human RFX1 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The RFX1 gene is a member of the regulatory factor X family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by the RFX1 gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes (provided by RefSeq, Jul 2008).
Rabbit anti Human RFX1 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RFX1 detects a band of approximately 140 kDa in Jurkat cell lysate
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 896-925 of human RFX1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P22670 Related reagents
- Entrez Gene
- RFX1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005634 nucleus
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0006955 immune response
- GO:0030528 transcription regulator activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RFX1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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