Retinoblastoma (pSer807) antibody

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Rabbit anti Retinoblastoma (pSer807)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2678 WB 50 µl
Rabbit anti retinoblastoma (pSer807) antibody recognizes retinoblastoma gene protein, also known as retinoblastoma-associated protein, p105-Rb, pp110 and Rb, when phosphorylated at serine 807. Retinoblastoma-associated protein is a tumor suppressor protein that plays a key role in G1 checkpoint control. In quiescent cells retinoblastoma-associated protein forms a repressor complex by binding to E2F transcription factors. Phosphorylation of retinoblastoma-associated protein results in dissociation from E2F, allowing for cell cycle progression.

Retinoblastoma-associated protein is phosphorylated at serine 807 by cyclin-D-cdk4/6, cdk9, cyclin C/Cdk3, Cdk5 and p38 MAPK kinase (Macdonald and Dick 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to retinoblastoma (pSer807) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 807 of human retinoblastoma

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P06400 Related reagents
Entrez Gene
RB1 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000279 M phase
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0006338 chromatin remodeling
GO:0006469 negative regulation of protein kinase activity
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0008134 transcription factor binding
GO:0016514 SWI/SNF complex
GO:0016564 transcription repressor activity
GO:0016605 PML body
GO:0019900 kinase binding
GO:0030308 negative regulation of cell growth
GO:0030521 androgen receptor signaling pathway
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031625 ubiquitin protein ligase binding
GO:0035189 Rb-E2F complex
GO:0043433 negative regulation of transcription factor activity
GO:0043550 regulation of lipid kinase activity
GO:0044419 interspecies interaction between organisms
GO:0045445 myoblast differentiation
GO:0045749 negative regulation of S phase of mitotic cell cycle
GO:0050681 androgen receptor binding
GO:0051219 phosphoprotein binding
Regulatory
For research purposes only

Applications of Retinoblastoma (pSer807) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Macdonald, J.I. & Dick, F.A. (2012) Posttranslational modifications of the retinoblastoma tumor suppressor protein as determinants of function.
    Genes Cancer. 3 (11-12): 619-33.
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