Retinoblastoma (pSer780) antibody
Rabbit anti retinoblastoma (pSer780) antibody recognizes retinoblastoma gene protein, also known as retinoblastoma-associated protein, p105-Rb, pp110 and Rb, when phosphorylated at serine 780. Retinoblastoma-associated protein is a tumor suppressor protein that plays a key role in G1 checkpoint control. In quiescent cells retinoblastoma-associated protein forms a repressor complex by binding to E2F transcription factors. Phosphorylation of retinoblastoma-associated protein results in dissociation from E2F, allowing for cell cycle progression.
Retinoblastoma-associated protein is phosphorylated at serine 780 by cyclin-D-cdk4/6 complexes, p38 MAPK kinase and aurora b kinase (Macdonald and Dick 2012).
Retinoblastoma-associated protein is phosphorylated at serine 780 by cyclin-D-cdk4/6 complexes, p38 MAPK kinase and aurora b kinase (Macdonald and Dick 2012).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to retinoblastoma (pSer780) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 780 of human retinoblastoma
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06400
- Entrez Gene
- RB1
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000279 M phase
- GO:0000785 chromatin
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0006338 chromatin remodeling
- GO:0006469 negative regulation of protein kinase activity
- GO:0007050 cell cycle arrest
- GO:0007265 Ras protein signal transduction
- GO:0008134 transcription factor binding
- GO:0016514 SWI/SNF complex
- GO:0016564 transcription repressor activity
- GO:0016605 PML body
- GO:0019900 kinase binding
- GO:0030308 negative regulation of cell growth
- GO:0030521 androgen receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031625 ubiquitin protein ligase binding
- GO:0035189 Rb-E2F complex
- GO:0043433 negative regulation of transcription factor activity
- GO:0043550 regulation of lipid kinase activity
- GO:0044419 interspecies interaction between organisms
- GO:0045445 myoblast differentiation
- GO:0045749 negative regulation of S phase of mitotic cell cycle
- GO:0050681 androgen receptor binding
- GO:0051219 phosphoprotein binding
- Regulatory
- For research purposes only
Applications of Retinoblastoma (pSer780) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Macdonald, J.I. & Dick, F.A. (2012) Posttranslational modifications of the retinoblastoma tumor suppressor protein as determinants of function.
Genes Cancer. 3 (11-12): 619-33.
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