Rabbit anti retinoblastoma (pSer780) antibody
recognizes retinoblastoma gene protein, also known as retinoblastoma-associated protein, p105-Rb, pp110 and Rb, when phosphorylated at serine 780. Retinoblastoma-associated protein is a tumor suppressor protein that plays a key role in G1
checkpoint control. In quiescent cells retinoblastoma-associated protein forms a repressor complex by binding to E2F transcription factors. Phosphorylation of retinoblastoma-associated protein results in dissociation from E2F, allowing for cell cycle progression.
Retinoblastoma-associated protein is phosphorylated at serine 780 by cyclin-D-cdk4/6 complexes, p38 MAPK kinase and aurora b kinase (Macdonald and Dick 2012
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to retinoblastoma (pSer780) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 780 of human retinoblastoma
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
G1 phase of mitotic cell cycle
regulation of transcription involved in G1/S phase of mitotic cell cycle
S phase of mitotic cell cycle
sequence-specific DNA binding transcription factor activity
transcription coactivator activity
negative regulation of protein kinase activity
cell cycle arrest
Ras protein signal transduction
transcription factor binding
transcription repressor activity
negative regulation of cell growth
androgen receptor signaling pathway
mitotic cell cycle G1/S transition checkpoint
ubiquitin protein ligase binding
negative regulation of transcription factor activity
regulation of lipid kinase activity
interspecies interaction between organisms
negative regulation of S phase of mitotic cell cycle
androgen receptor binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Retinoblastoma (pSer780) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Macdonald, J.I. & Dick, F.A. (2012) Posttranslational modifications of the retinoblastoma tumor suppressor protein as determinants of function.
Genes Cancer. 3 (11-12): 619-33.
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