Retinoblastoma (pSer780) antibody

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Rabbit anti Retinoblastoma (pSer780)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2676	WB			50 µl

Rabbit anti retinoblastoma (pSer780) antibody recognizes retinoblastoma gene protein, also known as retinoblastoma-associated protein, p105-Rb, pp110 and Rb, when phosphorylated at serine 780. Retinoblastoma-associated protein is a tumor suppressor protein that plays a key role in G₁ checkpoint control. In quiescent cells retinoblastoma-associated protein forms a repressor complex by binding to E2F transcription factors. Phosphorylation of retinoblastoma-associated protein results in dissociation from E2F, allowing for cell cycle progression.

Retinoblastoma-associated protein is phosphorylated at serine 780 by cyclin-D-cdk4/6 complexes, p38 MAPK kinase and aurora b kinase (Macdonald and Dick 2012).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to retinoblastoma (pSer780) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding serine 780 of human retinoblastoma

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P06400 Related reagents
Entrez Gene: RB1 Related reagents
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000279 M phase; GO:0000785 chromatin; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003713 transcription coactivator activity; GO:0006338 chromatin remodeling; GO:0006469 negative regulation of protein kinase activity; GO:0007050 cell cycle arrest; GO:0007265 Ras protein signal transduction; GO:0008134 transcription factor binding; GO:0016514 SWI/SNF complex; GO:0016564 transcription repressor activity; GO:0016605 PML body; GO:0019900 kinase binding; GO:0030308 negative regulation of cell growth; GO:0030521 androgen receptor signaling pathway; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031625 ubiquitin protein ligase binding; GO:0035189 Rb-E2F complex; GO:0043433 negative regulation of transcription factor activity; GO:0043550 regulation of lipid kinase activity; GO:0044419 interspecies interaction between organisms; GO:0045445 myoblast differentiation; GO:0045749 negative regulation of S phase of mitotic cell cycle; GO:0050681 androgen receptor binding; GO:0051219 phosphoprotein binding
Regulatory: For research purposes only

Applications of Retinoblastoma (pSer780) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB