Retinoblastoma-Binding Protein 5 antibody

Rabbit anti Retinoblastoma-Binding Protein 5

Product Type
PrecisionAb Polyclonal
Polyclonal IgG
Retinoblastoma-Binding Protein 5

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WB 100 µl loader
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Rabbit anti Human retinoblastoma-binding protein 5 antibody recognizes retinoblastoma-binding protein 5 (RBBP-5), also known as SWD1, Set1c WD40 repeat protein, homolog and retinoblastoma-binding protein RBQ-3.

Encoded by the RBBP5 gene, retinoblastoma-binding protein 5 is a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. Retinoblastoma-binding protein 5 binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for RBBP5 (provided by RefSeq, Jul 2010).

Rabbit anti Human retinoblastoma-binding protein 5 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Western Blotting
Anti retinoblastoma-binding protein 5 detects a band of approximately 67 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
KLH conjugated synthetic peptide between 451-485 amino acids from the C-terminal region of human retinoblastoma-binding protein 5
For research purposes only.
12 months from date of despatch.
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0006350 transcription
GO:0010843 promoter binding
GO:0043627 response to estrogen stimulus
GO:0045449 regulation of transcription
GO:0048188 Set1C/COMPASS complex
GO:0051568 histone H3-K4 methylation
GO:0071339 MLL1 complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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