Retinoblastoma-Binding Protein 5 antibody

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Rabbit anti Retinoblastoma-Binding Protein 5

Retinoblastoma-binding protein 5 antibody recognizes retinoblastoma-binding protein 5, also known as RBBP5, SWD1, Set1c WD40 repeat protein, homolog or retinoblastoma-binding protein RBQ-3. This antibody has been extensively validated for western blotting using whole cell lysates.

Retinoblastoma-Binding Protein 5 Antibody with Control Lysate

Retinoblastoma-binding protein 5 antibody recognizes retinoblastoma-binding protein 5, also known as RBBP5, SWD1, Set1c WD40 repeat protein, homolog or retinoblastoma-binding protein RBQ-3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00218 WB 100 µl
VPA00218KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human retinoblastoma-binding protein 5 antibody recognizes retinoblastoma-binding protein 5 (RBBP-5), also known as SWD1, Set1c WD40 repeat protein, homolog and retinoblastoma-binding protein RBQ-3.

Encoded by the RBBP5 gene, retinoblastoma-binding protein 5 is a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. Retinoblastoma-binding protein 5 binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for RBBP5 (provided by RefSeq, Jul 2010).

Rabbit anti Human retinoblastoma-binding protein 5 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti retinoblastoma-binding protein 5 detects a band of approximately 67 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 451-485 amino acids from the C-terminal region of human retinoblastoma-binding protein 5
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q15291 Related reagents
Entrez Gene
RBBP5 Related reagents
GO Terms
GO:0005515 protein binding
GO:0006350 transcription
GO:0010843 promoter binding
GO:0043627 response to estrogen stimulus
GO:0045449 regulation of transcription
GO:0048188 Set1C/COMPASS complex
GO:0051568 histone H3-K4 methylation
GO:0071339 MLL1 complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Retinoblastoma-Binding Protein 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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