Replication Factor C Subunit 4 antibody
Rabbit anti Replication Factor C Subunit 4
Replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.
Replication Factor C Subunit 4 Antibody with Control Lysate
Replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00770 | WB | 100 µl | ||||
| VPA00770KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits. Subunit 4 forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported (provided by RefSeq, Jul 2008).
Rabbit anti Human replication factor C subunit 4 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits. Subunit 4 forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported (provided by RefSeq, Jul 2008).
Rabbit anti Human replication factor C subunit 4 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti replication factor C subunit 4 antibody recognizes a band of approximately 40 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- TRIS buffered glycine
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- 0.01% Thiomersal
<10% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
<10% Glycerol - Immunogen
- Recombinant protein encompassing a sequence within the center region of human replication factor C subunit 4
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P35249 Related reagents
- Entrez Gene
- RFC4 Related reagents
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000722 telomere maintenance via recombination
- GO:0003689 DNA clamp loader activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005663 DNA replication factor C complex
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032201 telomere maintenance via semi-conservative replication
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Replication Factor C Subunit 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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