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Replication Factor C Subunit 4 antibody

Rabbit anti Replication Factor C Subunit 4

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Replication Factor C Subunit 4

Product Code Applications Pack Size List Price Your Price Qty
VPA00770
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Rabbit anti Human replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits. Subunit 4 forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported (provided by RefSeq, Jul 2008).

Rabbit anti Human replication factor C subunit 4 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti replication factor C subunit 4 antibody recognizes a band of approximately 40 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Immunogen
Recombinant protein encompassing a sequence within the center region of human replication factor C subunit 4
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P35249
Entrez Gene
RFC4
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0003689 DNA clamp loader activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032201 telomere maintenance via semi-conservative replication

VPA00770

170504

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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