Replication Factor C Subunit 4 antibody

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Rabbit anti Replication Factor C Subunit 4

Replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.

Replication Factor C Subunit 4 Antibody with Control Lysate

Replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00770 WB 100 µl
VPA00770KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human replication factor C subunit 4 antibody recognizes replication factor C subunit 4, also known as A1 37 kDa subunit, RF-C 37 kDa subunit, activator 1 subunit 4 or replication factor C 37 kDa subunit.

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits. Subunit 4 forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported (provided by RefSeq, Jul 2008).

Rabbit anti Human replication factor C subunit 4 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti replication factor C subunit 4 antibody recognizes a band of approximately 40 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
TRIS buffered glycine
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<10% Glycerol
Immunogen
Recombinant protein encompassing a sequence within the center region of human replication factor C subunit 4
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35249 Related reagents
Entrez Gene
RFC4 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0003689 DNA clamp loader activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032201 telomere maintenance via semi-conservative replication
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Replication Factor C Subunit 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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