RBBP4 antibody
Rabbit anti RBBP4
Rabbit anti human RBBP4 antibody recognizes the histone-binding protein RBBP4, also known as RbAp48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, MSI1 protein homolog, or retinoblastoma-binding protein p48. This antibody has been extensively validated for western blotting.
RBBP4 Antibody with Control Lysate
Rabbit anti human RBBP4 antibody recognizes the histone-binding protein RBBP4, also known as RbAp48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, MSI1 protein homolog, chromatin assembly factor/CAF-1 p48 subunit, or nucleosome-remodeling factor subunit RBAP48.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00333 | WB | 100 µl | ||||
| VPA00333KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RBBP4 antibody recognizes RBBP4, also known as RbAp48, CAF-1 subunit C, CAF-I 48 kDa subunit, chromatin assembly factor 1 subunit C, nucleosome-remodeling factor subunit RBAP48, retinoblastoma-binding protein 4 and retinoblastoma-binding protein p48.
The RBBP4 gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for RBBP4 (provided by RefSeq, Sep 2008).
Rabbit anti Human RBBP4 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The RBBP4 gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for RBBP4 (provided by RefSeq, Sep 2008).
Rabbit anti Human RBBP4 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RBBP4 detects a band of approximately 52 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the N-terminal region of human RBBP4
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q09028 Related reagents
- Entrez Gene
- RBBP4 Related reagents
- GO Terms
- GO:0006260 DNA replication
- GO:0042393 histone binding
- GO:0006350 transcription
- GO:0007049 cell cycle
- GO:0008285 negative regulation of cell proliferation
- GO:0016580 Sin3 complex
- GO:0016581 NuRD complex
- GO:0016589 NURF complex
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0035098 ESC/E(Z) complex
- GO:0042826 histone deacetylase binding
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RBBP4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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