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RBBP4 antibody

Rabbit anti RBBP4

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
RBBP4

Product Code Applications Pack Size List Price Your Price Qty
VPA00333
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WB 100 µl loader
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Rabbit anti Human RBBP4 antibody recognizes RBBP4, also known as RbAp48, CAF-1 subunit C, CAF-I 48 kDa subunit, chromatin assembly factor 1 subunit C, nucleosome-remodeling factor subunit RBAP48, retinoblastoma-binding protein 4 and retinoblastoma-binding protein p48.

The RBBP4 gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for RBBP4 (provided by RefSeq, Sep 2008).

Rabbit anti Human RBBP4 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RBBP4 detects a band of approximately 52 kDa in HEK293 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the N-terminal region of human RBBP4
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q09028
Entrez Gene
RBBP4
GO Terms
GO:0006260 DNA replication
GO:0042393 histone binding
GO:0006350 transcription
GO:0007049 cell cycle
GO:0008285 negative regulation of cell proliferation
GO:0016580 Sin3 complex
GO:0016581 NuRD complex
GO:0016589 NURF complex
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0035098 ESC/E(Z) complex
GO:0042826 histone deacetylase binding
GO:0045449 regulation of transcription

VPA00333

160411

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