RAR Beta antibody

100% Secure


Rabbit anti Human RAR Beta (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1138 P* 50 µg
Rabbit anti Human RAR beta antibody detects an epitope within the C-terminal (CT) region of retinoic acid receptor beta (RAR beta), a ~55 kDa transcriptional factor activated by retinoic acid. Once activated RAR beta heterodimerizes with retinoid X receptors (RXRs) and translocates to the nucleus, where it stimulates the transcription of growth-inhibitory, differentiation-inducing genes. In this way RAR beta mediates cellular signalling in embryonic morphogenesis and organogenesis.

The RAR beta exists as three isoforms, which are localised to the cytoplasm or subnuclear compartments. Down-regulation of the RAR-b2 isoform occurs early in tumorigenesis, and is a tumour suppressor gene.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to RAR beta were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the C-terminal region of human RAR beta.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10826 Related reagents
Entrez Gene
RARB Related reagents
GO Terms
GO:0005515 protein binding
GO:0003707 steroid hormone receptor activity
GO:0003708 retinoic acid receptor activity
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0008270 zinc ion binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
Regulatory
For research purposes only

Applications of RAR Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 15ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Intermediate trophoblasts.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for RAR Beta antibody

  1. Swift, M.E. et al. (2006) Truncated RAR beta isoform enhances proliferation and retinoid resistance.
    J Cell Physiol. 209 (3): 718-25.
  2. Desai, T.J. et al. (2006) Distinct roles for retinoic acid receptors alpha and beta in early lung morphogenesis.
    Dev Biol. 291 (1): 12-24.
  3. Grunt, T.W. et al. (2005) An EGF receptor inhibitor induces RAR-beta expression in breast and ovarian cancer cells.
    Biochem Biophys Res Commun. 329 (4): 1253-9.