RAP1A antibody

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Rabbit anti RAP1A

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00926
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human RAP1A antibody recognizes the Ras-related protein Rap-1A, also known as GTP-binding protein smg p21A and Ras-related protein Krev-1.

The product of RAP1A belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of RAP1A counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for RAP1A (provided by RefSeq, Jul 2008).

Rabbit anti Human RAP1A antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RAP1A antibody recognizes a band of approximately 21 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Synthetic peptide of human RAP1A

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P62834 Related reagents
Entrez Gene
RAP1A Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0003924 GTPase activity
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0006112 energy reserve metabolic process
GO:0050796 regulation of insulin secretion
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RAP1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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