RAP1 antibody

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Rabbit anti RAP1

Rabbit anti human RAP1 antibody recognizes telomeric repeat-binding factor 2-interacting protein 1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, or dopamine receptor interacting protein 5. This antibody has been validated for western blotting using whole cell lysates.

RAP1 Antibody with Control Lysate

Rabbit anti human RAP1 antibody recognizes telomeric repeat-binding factor 2-interacting protein 1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein or dopamine receptor interacting protein 5.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00341 WB 100 µl
VPA00341KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human RAP1 antibody recognizes RAP1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein, dopamine receptor interacting protein 5, hRap1, telomeric repeat binding factor 2 interacting protein and repressor/activator protein 1 homolog.

The RAP1 gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22 (provided by RefSeq, Apr 2010).

Rabbit anti Human RAP1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RAP1 detects a band of approximately 50 kDa in HeLa cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 349-378 of human RAP1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9NYB0 Related reagents
Entrez Gene
TERF2IP Related reagents
GO Terms
GO:0005515 protein binding
GO:0000783 nuclear telomere cap complex
GO:0003677 DNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006350 transcription
GO:0007004 telomere maintenance via telomerase
GO:0010569 regulation of double-strand break repair via homologous recombination
GO:0030870 Mre11 complex
GO:0031848 protection from non-homologous end joining at telomere
GO:0032205 negative regulation of telomere maintenance
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0048239 negative regulation of DNA recombination at telomere
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0070198 protein localization to chromosome, telomeric region
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RAP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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