RAP1 antibody
Rabbit anti RAP1
Rabbit anti human RAP1 antibody recognizes telomeric repeat-binding factor 2-interacting protein 1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, or dopamine receptor interacting protein 5. This antibody has been validated for western blotting using whole cell lysates.
RAP1 Antibody with Control Lysate
Rabbit anti human RAP1 antibody recognizes telomeric repeat-binding factor 2-interacting protein 1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein or dopamine receptor interacting protein 5.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00341 | WB | 100 µl | ||||
| VPA00341KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RAP1 antibody recognizes RAP1, also known as RAP1 homolog, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein, dopamine receptor interacting protein 5, hRap1, telomeric repeat binding factor 2 interacting protein and repressor/activator protein 1 homolog.
The RAP1 gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22 (provided by RefSeq, Apr 2010).
Rabbit anti Human RAP1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The RAP1 gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22 (provided by RefSeq, Apr 2010).
Rabbit anti Human RAP1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RAP1 detects a band of approximately 50 kDa in HeLa cell lysate
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 349-378 of human RAP1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9NYB0 Related reagents
- Entrez Gene
- TERF2IP Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000783 nuclear telomere cap complex
- GO:0003677 DNA binding
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0006350 transcription
- GO:0007004 telomere maintenance via telomerase
- GO:0010569 regulation of double-strand break repair via homologous recombination
- GO:0030870 Mre11 complex
- GO:0031848 protection from non-homologous end joining at telomere
- GO:0032205 negative regulation of telomere maintenance
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0048239 negative regulation of DNA recombination at telomere
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0070198 protein localization to chromosome, telomeric region
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RAP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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