RAF-1 (pSer259) antibody
Rabbit anti RAF-1 (pSer259)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- RAF-1 (pSer259)
Antibody Quality and 
Unrivaled 
| Rabbit anti RAF-1 (pSer259) antibody recognizes RAF-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 259. RAF-1 is a serine/threonine protein kinase and a member of the RAF family. Upon activation by Ras, RAF-1 initiates the MAPK signaling cascade, playing a role in regulation of cell proliferation, differentiation, survival and apoptosis. RAF-1 is phosphorylated by AKT at serine 259. Phosphorylation of RAF-1 at serine 259 allows binding of 14-3-3 proteins, inhibiting the activity of RAF-1 (Kubicek et al. 2002). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to RAF-1 (pSer259) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 259 of human RAF-1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
 |
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Kubicek, M. et al. (2002) Dephosphorylation of Ser-259 regulates Raf-1 membrane association.
J Biol Chem. 277 (10): 7913-9.
- UniProt
- P04049
- Entrez Gene
- RAF1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000186 activation of MAPKK activity
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005057 receptor signaling protein activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- View More GO Terms
- GO:0005741 mitochondrial outer membrane
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007268 synaptic transmission
- GO:0007411 axon guidance
- GO:0008283 cell proliferation
- GO:0008285 negative regulation of cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0030168 platelet activation
- GO:0048011 nerve growth factor receptor signaling pathway
View more products with RAF-1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up