Rabbit anti RAF-1 (pSer259) antibody
recognizes RAF-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 259. RAF-1 is a serine/threonine protein kinase and a member of the RAF family. Upon activation by Ras, RAF-1 initiates the MAPK signaling cascade, playing a role in regulation of cell proliferation, differentiation, survival and apoptosis. RAF-1 is phosphorylated by AKT at serine 259. Phosphorylation of RAF-1 at serine 259 allows binding of 14-3-3 proteins, inhibiting the activity of RAF-1 (Kubicek et al. 2002
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to RAF-1 (pSer259) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 259 of human RAF-1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPKK activity
protein serine/threonine kinase activity
receptor signaling protein activity
metal ion binding
mitochondrial outer membrane
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
negative regulation of cell proliferation
insulin receptor signaling pathway
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of RAF-1 (pSer259) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Kubicek, M. et al. (2002) Dephosphorylation of Ser-259 regulates Raf-1 membrane association.
J Biol Chem. 277 (10): 7913-9.
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