Radixin antibody

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Goat anti Radixin

Radixin antibody recognizes radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. The antibody has been extensively validated for western blotting using whole cell lysates.

Radixin Antibody with Control Lysate

Radixin antibody recognizes radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00052 WB 100 µl
VPA00052KT WB 2 Westerns
With Lysate 400µg
Goat anti Human radixin antibody recognizes human radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. Radixin is a member of the ezrin, radixin, moesin (ERM) protein family. It is involved in cytoskeletal anchoring playing a crucial role as a linker protein between the plasma membrane and the barbed end of the actin cytoskeleton (Salles et al. 2013).

Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).

Goat anti Human radixin recognizes radixin as a single band of 80 kDa in a range of human cell line lysates tested cell lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti radixin detects a band of approximately 80 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QARDETKKTQND from the internal region of radixin.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35241 Related reagents
Entrez Gene
RDX Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003779 actin binding
GO:0005730 nucleolus
GO:0005794 Golgi apparatus
GO:0005856 cytoskeleton
GO:0019898 extrinsic to membrane
GO:0032154 cleavage furrow
GO:0051693 actin filament capping
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Radixin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

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