Radixin antibody
Goat anti Radixin
Radixin antibody recognizes radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. The antibody has been extensively validated for western blotting using whole cell lysates.
Radixin Antibody with Control Lysate
Radixin antibody recognizes radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00052 | WB | 100 µl | ||||
| VPA00052KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human radixin antibody recognizes human radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. Radixin is a member of the ezrin, radixin, moesin (ERM) protein family. It is involved in cytoskeletal anchoring playing a crucial role as a linker protein between the plasma membrane and the barbed end of the actin cytoskeleton (Salles et al. 2013).
Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).
Goat anti Human radixin recognizes radixin as a single band of 80 kDa in a range of human cell line lysates tested cell lysates. Cross reactivity with rodent cell lines is also observed.
Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).
Goat anti Human radixin recognizes radixin as a single band of 80 kDa in a range of human cell line lysates tested cell lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti radixin detects a band of approximately 80 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QARDETKKTQND from the internal region of radixin.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P35241 Related reagents
- Entrez Gene
- RDX Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0003779 actin binding
- GO:0005730 nucleolus
- GO:0005794 Golgi apparatus
- GO:0005856 cytoskeleton
- GO:0019898 extrinsic to membrane
- GO:0032154 cleavage furrow
- GO:0051693 actin filament capping
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Radixin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
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