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Radixin antibody

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Western Blot Validated
Anti Radixin Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with radixin antibody followed by detection with HRP conjugated Rabbit anti Goat IgG (Fc) (1/10,000, STAR122P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to radixin (molecular weight 80 kDa)

Goat anti Radixin

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00052
Datasheet
WB
MSDS
100 µl

Goat anti Human radixin antibody recognizes human radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain. Radixin is a member of the ezrin, radixin, moesin (ERM) protein family. It is involved in cytoskeletal anchoring playing a crucial role as a linker protein between the plasma membrane and the barbed end of the actin cytoskeleton (Salles et al. 2013).

Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).

Goat anti Human radixin recognizes radixin as a single band of 80 kDa in a range of human cell line lysates tested cell lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti radixin detects a band of approximately 80 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QARDETKKTQND from the internal region of radixin.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P35241
Entrez Gene
RDX
GO Terms
GO:0005886 plasma membrane
GO:0003779 actin binding
GO:0005730 nucleolus
GO:0005794 Golgi apparatus
GO:0005856 cytoskeleton
GO:0019898 extrinsic to membrane
GO:0032154 cleavage furrow
GO:0051693 actin filament capping
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Radixin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the Radixin specificity