RAC1 (pSer71) antibody

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Rabbit anti RAC1 (pSer71)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2673 WB 50 µl
Rabbit anti RAC1 (pSer71) antibody recognizes RAC1, also known as ras-related C3 botulinum toxin substrate 1, cell migration-inducing gene 5 protein, ras-like protein TC25 or p21-Rac1, when phosphorylated at serine 71. RAC1 is a member of the small Rho GTPase family that plays a role in numerous cell processes including cell migration, cell polarization, apoptosis and gene expression. RAC1 cycles between an active GTP-bound state and an inactive GDP-bound state. RAC1 is phosphorylated at serine 71 by Akt. Phosphorylation at serine 71 modulates RAC1 signaling by directing interaction with specific signaling pathways (Schwarz et al. 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to RAC1 (pSer71) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 71 of human RAC1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P63000 Related reagents
Entrez Gene
RAC1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0007160 cell-matrix adhesion
GO:0007264 small GTPase mediated signal transduction
GO:0007411 axon guidance
GO:0008624 induction of apoptosis by extracellular signals
GO:0030041 actin filament polymerization
GO:0016032 viral reproduction
GO:0010310 regulation of hydrogen peroxide metabolic process
GO:0010592 positive regulation of lamellipodium assembly
GO:0030032 lamellipodium assembly
GO:0030168 platelet activation
GO:0030742 GTP-dependent protein binding
GO:0031295 T cell costimulation
GO:0031529 ruffle organization
GO:0031996 thioesterase binding
GO:0032707 negative regulation of interleukin-23 production
GO:0035025 positive regulation of Rho protein signal transduction
GO:0042470 melanosome
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048261 negative regulation of receptor-mediated endocytosis
GO:0050690 regulation of defense response to virus by virus
GO:0051668 localization within membrane
GO:0060263 regulation of respiratory burst
Regulatory
For research purposes only

Applications of RAC1 (pSer71) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Schwarz, J. et al. (2012) Serine-71 phosphorylation of Rac1 modulates downstream signaling.
    PLoS One. 7 (9): e44358.