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RAB8A antibody

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Anti Human RAB8A (C-Terminal) Antibody thumbnail image 1
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Western blotting analysis of human cerebellum lysate (35μg protein in RIPA buffer) probed with Goat anti Human RAB8A antibody (AHP1683) used at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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Goat anti Human RAB8A (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RAB8A
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1683
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price

Goat anti Human RAB8A antibody recognizes an epitope within the C-terminal (CT) region of the human RAS superfamily member Rab8a (Ras-related protein Rab-8A), otherwise known as oncogene c-mel, Rab8A is a small monomeric GTP/GDP-binding protein with a predicted molecular weight of ~24 kDa which may play a role in vesicular trafficking and neurotransmitter release.

The Rab family of proteins, originally described as Ras-like proteins in brain are the largest group of GTPases within the Ras superfamily with 61 protein members identified. Rab family members are involved in the regulation of intracellular vesicular transport and movement of proteins between endocytic and secretory organelles (Wennerberg et al. 2005). Rab8A is involved in vesicular traffic between the trans golgi network and the basoleteral aspect of the plasma membrane (Huber et al. 1993).

Goat anti human Rab8a antibody has been successfully used for the detection of Rab8a in human intestinal epithelial cells using western blotting (Vacca et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human RAB8A (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-NQGVKITPDQQKR from the C-Terminal region of RAB8A (NP_005361.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Western Blotting 0.1 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1683 detects a band of approximately 25kDa in human cerebellum and kidney cell lysates

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for RAB8A antibody

  1. Vacca, B. et al. (2014) Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function.
    Hum Mol Genet. 23 (11): 2834-46.

Synonyms
c-mel
RRID
AB_2175309
UniProt
P61006
Entrez Gene
RAB8A
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0005794 Golgi apparatus
GO:0006904 vesicle docking involved in exocytosis
GO:0007264 small GTPase mediated signal transduction
GO:0015031 protein transport
GO:0031513 nonmotile primary cilium
GO:0042384 cilium assembly
GO:0048210 Golgi vesicle fusion to target membrane

AHP1683

146394 146899

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