PTRF antibody

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PTRF Antibody with Control Lysate

Rabbit anti PTRF

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00574KT WB 2 Westerns
VPA00574 WB 100 µl
Rabbit anti Human PTRF antibody recognizes PTRF also known as polymerase I and transcript release factor, TTF-I interacting peptide 12 or cavin-1.

The PTRF gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in PTRF result in CGL4, a disorder characterized by generalized lipodystrophy and muscular dystrophy (Hayashi et al. 2009) (provided by RefSeq, Nov 2009).

Rabbit anti Human PTRF antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 1-30 of human PTRF

Storage Information

Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q6NZI2 Related reagents
Entrez Gene
PTRF Related reagents
GO Terms
GO:0005515 protein binding
GO:0003716 RNA polymerase I transcription termination factor activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005654 nucleoplasm
GO:0005792 microsome
GO:0005901 caveola
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0042134 rRNA primary transcript binding
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PTRF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti PTRF detects a band of approximately 60 kDa in A549 cell lysates
Western Blotting
Anti PTRF detects a band of approximately 60 kDa in A549 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB