PTRF antibody

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Rabbit anti PTRF

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00574
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PTRF antibody recognizes PTRF also known as polymerase I and transcript release factor, TTF-I interacting peptide 12 or cavin-1.

The PTRF gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in PTRF result in CGL4, a disorder characterized by generalized lipodystrophy and muscular dystrophy (Hayashi et al. 2009) (provided by RefSeq, Nov 2009).

Rabbit anti Human PTRF antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PTRF detects a band of approximately 60 kDa in A549 cell lysates.
Product Form
Purified IgG - liquid.
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 1-30 of human PTRF

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6NZI2
Entrez Gene
PTRF
GO Terms
GO:0005515 protein binding
GO:0003716 RNA polymerase I transcription termination factor activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005654 nucleoplasm
GO:0005792 microsome
GO:0005901 caveola
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0042134 rRNA primary transcript binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of PTRF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml