PTPN6 antibody

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Rabbit anti PTPN6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2518 IF WB 50 µl
Rabbit anti PTPN6 antibody recognizes tyrosine-protein phosphatase non-receptor type 6 (PTPN6) also known as hematopoietic cell protein tyrosine phosphatase. PTPN6 belongs to the protein tyrosine phosphatase family and interacts with surface receptors such as CD117 and epidermal growth factor receptor (EGFR) which have undergone tyrosine phosphorylation.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PTPN6 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human PTPN6

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P29350 Related reagents
Entrez Gene
PTPN6 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004725 protein tyrosine phosphatase activity
GO:0005634 nucleus
GO:0006470 protein dephosphorylation
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0034329 cell junction assembly
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050900 leukocyte migration
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
GO:2000045 regulation of G1/S transition of mitotic cell cycle
Regulatory
For research purposes only

Applications of PTPN6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti PTPN6 antibody detects a band of approximately 65 kDa in cell lysates and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*