PTPN11 (pTyr542) antibody
Filter by Application:
WB P ResetRabbit anti PTPN11 (pTyr542)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- PTPN11 (pTyr542)
Antibody Quality and Performance Guarantee
| Rabbit anti PTPN11 (pTyr542) antibody recognizes PTPN11 also known as tyrosine-protein phosphatase non-receptor type 11, PTP2C or SHPTP2, when phosphorylated at tyrosine 542. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. PTPN11 contains two Src homology-2 domains which bind phospho-tyrosine domains. PTPs regulate numerous cellular functions including cell proliferation and oncogenic transformation. EphA2 has been identified as a kinase that phosphorylates PTPN11 at tyrosine 542 leading to the recruitment of Grb2 (Miura et al. 2013). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PTPN11 (pTyr542) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 542 of human PTPN11
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/50 | 1/100 |
| Western Blotting | |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
 |
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Miura, K. et al. (2013) Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase.
Oncogene. 32 (45): 5292-301.
- UniProt
- Q06124
- Entrez Gene
- PTPN11
- GO Terms
- GO:0005829 cytosol
- GO:0004726 non-membrane spanning protein tyrosine phosphatase activity
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007411 axon guidance
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0034329 cell junction assembly
- View More GO Terms
- GO:0050900 leukocyte migration
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- GO:0060338 regulation of type I interferon-mediated signaling pathway
AHP2672
141629
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up