PTPN11 (pTyr542) antibody

Rabbit anti PTPN11 (pTyr542)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PTPN11 (pTyr542)

Product Code Applications Pack Size List Price Your Price Qty
AHP2672
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SDS Safety Datasheet SDS
P WB 50 µl loader
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Rabbit anti PTPN11 (pTyr542) antibody recognizes PTPN11 also known as tyrosine-protein phosphatase non-receptor type 11, PTP2C or SHPTP2, when phosphorylated at tyrosine 542. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. PTPN11 contains two Src homology-2 domains which bind phospho-tyrosine domains. PTPs regulate numerous cellular functions including cell proliferation and oncogenic transformation. EphA2 has been identified as a kinase that phosphorylates PTPN11 at tyrosine 542 leading to the recruitment of Grb2 (Miura et al. 2013).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PTPN11 (pTyr542) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 542 of human PTPN11
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Miura, K. et al. (2013) Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase.
    Oncogene. 32 (45): 5292-301.

UniProt
Q06124
Entrez Gene
PTPN11
GO Terms
GO:0005829 cytosol
GO:0004726 non-membrane spanning protein tyrosine phosphatase activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0034329 cell junction assembly
GO:0050900 leukocyte migration
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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