Rabbit anti PTK2B (pTyr402) antibody
recognizes protein-tyrosine kinase 2-beta, also known as CADTK, CAKB, FADK 2, PYK2, when phosphorylated at tyrosine 402. It is a non-receptor tyrosine kinase that regulates cell migration and mediates transfer of signals from the cell surface to the cytoskeleton (Duong et al. 2001
, Avraham et al. 2000
). Lysophosphatidic acid (LPA) and bradykinin induce tyrosine phosphorylation of PTK2B (Dikic et al. 1996
). Phosphorylation of PTK2B on tyrosine 402 is important for binding with SH2 domain of SRC (Dikic et al. 1996). In Jurkat T cells, tyrosine 402 is involved in interleukin-2 production (Katagiri et al. 2000
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PTK2B (pTyr402) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- A phospho speciﬁc peptide corresponding to residues surrounding tyrosine 402 of human PTK2B
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
non-membrane spanning protein tyrosine kinase activity
signal transducer activity
signal complex assembly
positive regulation of cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PTK2B (pTyr402) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti PTK2B (pTyr402) antibody detects a band of approximately 116 kDa in HEK293 cell lysate under reducing conditions
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Secondary Antibodies Available