PTK2B (pTyr402) antibody

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Rabbit anti PTK2B (pTyr402)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2743
Datasheet
WB
SDS
50 µl loader

Rabbit anti PTK2B (pTyr402) antibody recognizes protein-tyrosine kinase 2-beta, also known as CADTK, CAKB, FADK 2, PYK2, when phosphorylated at tyrosine 402. It is a non-receptor tyrosine kinase that regulates cell migration and mediates transfer of signals from the cell surface to the cytoskeleton (Duong et al. 2001, Avraham et al. 2000). Lysophosphatidic acid (LPA) and bradykinin induce tyrosine phosphorylation of PTK2B (Dikic et al. 1996). Phosphorylation of PTK2B on tyrosine 402 is important for binding with SH2 domain of SRC (Dikic et al. 1996). In Jurkat T cells, tyrosine 402 is involved in interleukin-2 production (Katagiri et al. 2000).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PTK2B (pTyr402) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A phospho specific peptide corresponding to residues surrounding tyrosine 402 of human PTK2B

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14289
Entrez Gene
PTK2B
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0007172 signal complex assembly
GO:0008284 positive regulation of cell proliferation
Regulatory
For research purposes only

Applications of PTK2B (pTyr402) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti PTK2B (pTyr402) antibody detects a band of approximately 116 kDa in HEK293 cell lysate under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg