PTBP1 antibody

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Goat anti Human PTBP1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2067 E WB 0.1 mg
Goat anti Human PTBP1 antibody; recognizes an epitope within the N-terminal (NT) region of human PTBP1, otherwise known as polypyrimidine tract-binding protein 1. PTPB1 is a multi-functional, ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP), implicated in pre-mRNA processing, mRNA metabolism and transport. PTPB1 contains 4 RRM motifs.

PTBP1 is highly over-expressed in glioma and, along with its brain-specific homologue PTBP2, acts as a regulator of neural precursor cell differentiation (Wang et al. 2013). There are 3 described isoforms of PTPB1 derived by alternative splicing, isoforms differ in having insertions between the RRM domains 2 and 3. Goat anti Human PTBP1 antibody is expected to recognize all isoforms of PTPB1.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Bovine Expected from Sequence
Mouse Expected from Sequence
Rat Expected from Sequence
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human PTBP1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence DGIVPDIAVGTKR-C from the N-Terminal region of PTBP1 (NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P26599 Related reagents
Entrez Gene
PTBP1 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0008187 poly-pyrimidine tract binding
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
Regulatory
For research purposes only

Applications of PTBP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Western Blotting 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2067 detects a band of approximately 60-65kDa in Jurkat cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB