PSME3 antibody

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Rabbit anti PSME3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00714
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PSME3 antibody recognizes the proteasome activator complex subunit 3, also known as 11S regulator complex gamma subunit, PA28gamma, Ki antigen or activator of multicatalytic protease subunit 3.

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants (provided by RefSeq, May 2012).

Rabbit anti Human PSME3 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PSME3 detects a band of approximately 30 kDa in Ramos cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the N terminal region of human PSME3

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P61289-2
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PSME3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml