PSMD3 antibody
Rabbit anti Human PSMD3 antibody recognizes PSMD3, also known as 26S proteasome non-ATPase regulatory subunit 3 and tissue specific transplantation antigen 2.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The PSMD3 gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in PSMD3 are associated with neutrophil count (provided by RefSeq, Jul 2012).
Rabbit anti Human PSMD3 antibody detects a band of 61 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The PSMD3 gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in PSMD3 are associated with neutrophil count (provided by RefSeq, Jul 2012).
Rabbit anti Human PSMD3 antibody detects a band of 61 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PSMD3 detects a band of approximately 61 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the N-terminal region of human PSMD3
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43242
- Entrez Gene
- PSMD3
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0000502 proteasome complex
- GO:0006915 apoptosis
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0016032 viral reproduction
- GO:0016071 mRNA metabolic process
- GO:0042981 regulation of apoptosis
- GO:0030234 enzyme regulator activity
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0042176 regulation of protein catabolic process
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PSMD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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