PSMD3 antibody

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Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PSMD3 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to PSMD3 (molecular weight 61 kDa)

Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD3 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti PSMD3 antibody (**VPA00413**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 165 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti PSMD3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of PSMD3 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD3 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Rabbit anti PSMD3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PSMD3

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00413 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human PSMD3 antibody recognizes PSMD3, also known as 26S proteasome non-ATPase regulatory subunit 3 and tissue specific transplantation antigen 2.

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The PSMD3 gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in PSMD3 are associated with neutrophil count (provided by RefSeq, Jul 2012).

Rabbit anti Human PSMD3 antibody detects a band of 61 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PSMD3 detects a band of approximately 61 kDa in HEK293 cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide corresponding to the N-terminal region of human PSMD3
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: O43242
Entrez Gene: PSMD3
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000502 proteasome complex; GO:0006915 apoptosis; GO:0006521 regulation of cellular amino acid metabolic process; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0016032 viral reproduction; View More GO Terms; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0030234 enzyme regulator activity; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0042176 regulation of protein catabolic process; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle