PSMD14 antibody

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Anti PSMD14 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PSMD14 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to PSMD14 (molecular weight 35 kDa).

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Rabbit anti PSMD14

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PSMD14

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VPA00493 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human PSMD14 antibody recognizes PSMD14 also known as 26S proteasome non-ATPase regulatory subunit 14, POH1, 26S proteasome regulatory subunit rpn11 or 26S proteasome-associated PAD1 homolog 1.

The PSMD14 gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of PSMD14 is also located on the long arm of chromosome 2 (provided by RefSeq, Feb 2012).

Rabbit anti Human PSMD14 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PSMD14 detects a band of approximately 35 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the C terminal region of human PSMD14
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: O00487
Entrez Gene: PSMD14
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000502 proteasome complex; GO:0006915 apoptosis; GO:0004221 ubiquitin thiolesterase activity; GO:0008237 metallopeptidase activity; GO:0046872 metal ion binding; GO:0006521 regulation of cellular amino acid metabolic process; View More GO Terms; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0061133 endopeptidase activator activity; GO:0061136 regulation of proteasomal protein catabolic process; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0070536 protein K63-linked deubiquitination; GO:0070628 proteasome binding