PSMD14 antibody

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Rabbit anti PSMD14

Rabbit anti-human PSMD14 antibody recognizes the 26S proteasome non-ATPase regulatory subunit 14, also known as 26S proteasome regulatory subunit RPN11 or 26S proteasome-associated PAD1 homolog 1.

PSMD14 Antibody with Control Lysate

Rabbit anti-human PSMD14 antibody recognizes the 26S proteasome non-ATPase regulatory subunit 14, also known as 26S proteasome regulatory subunit RPN11 or 26S proteasome-associated PAD1 homolog 1.

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00493	WB	100 µl
VPA00493KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human PSMD14 antibody recognizes PSMD14 also known as 26S proteasome non-ATPase regulatory subunit 14, POH1, 26S proteasome regulatory subunit rpn11 or 26S proteasome-associated PAD1 homolog 1.

The PSMD14 gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of PSMD14 is also located on the long arm of chromosome 2 (provided by RefSeq, Feb 2012).

Rabbit anti Human PSMD14 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti PSMD14 detects a band of approximately 35 kDa in K562 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid.

Product Form

Purified IgG - liquid.

Preparation

Rabbit polyclonal antibody purified by affinity chromatography.

Preparation

20μl Rabbit polyclonal antibody purified by affinity chromatography.

Buffer Solution

Phosphate buffered saline.

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
2% Sucrose.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
2% Sucrose.

Immunogen

Synthetic peptide directed towards the C terminal region of human PSMD14

Instructions For Use

Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: O00487 Related reagents
Entrez Gene: PSMD14 Related reagents
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000502 proteasome complex; GO:0006915 apoptosis; GO:0004221 ubiquitin thiolesterase activity; GO:0008237 metallopeptidase activity; GO:0046872 metal ion binding; GO:0006521 regulation of cellular amino acid metabolic process; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0061133 endopeptidase activator activity; GO:0061136 regulation of proteasomal protein catabolic process; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0070536 protein K63-linked deubiquitination; GO:0070628 proteasome binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only.

Applications of PSMD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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