PSMB3 antibody

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Rabbit anti PSMB3

Rabbit anti human PSMB3 antibody recognizes proteasome subunit beta type-3, also known as proteasome chain 13, proteasome component C10-II, HC10-11 or proteasome theta chain. This antibody has been extensively validated for western blotting using whole cell lysates.

PSMB3 Antibody with Control Lysate

Rabbit anti human PSMB3 antibody recognizes proteasome subunit beta type-3, also known as proteasome chain 13, proteasome component C10-II, HC10-11 or proteasome theta chain. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00411 WB 100 µl
VPA00411KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PSMB3 antibody recognizes the PSMB3, also known as proteasome subunit beta type-3, HC10-11 and proteasome theta chain.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB3 encodes a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit. The 26S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants (provided by RefSeq, Sep 2013).

Rabbit anti Human PSMB3 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PSMB3 detects a band of approximately 25 kDa in HeLa cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the central region of human PSMB3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P49720 Related reagents
Entrez Gene
PSMB3 Related reagents
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0006915 apoptosis
GO:0004298 threonine-type endopeptidase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005839 proteasome core complex
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0016032 viral reproduction
GO:0016071 mRNA metabolic process
GO:0042981 regulation of apoptosis
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PSMB3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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