PRPF8 antibody
Rabbit anti PRPF8
Rabbit anti-human PRPF8 antibody recognizes pre-mRNA-processing-splicing factor 8, also known as 220 kDa U5 snRNP-specific protein, PRP8 homolog, ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1 or apoptosis-regulated protein 2, p220.
PRPF8 Antibody with Control Lysate
Rabbit anti-human PRPF8 antibody recognizes pre-mRNA-processing-splicing factor 8, also known as 220 kDa U5 snRNP-specific protein, PRP8 homolog, ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1 or apoptosis-regulated protein 2, p220.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00580 | WB | 100 µl | ||||
| VPA00580KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PRPF8 antibody recognizes PRPF8 also known as pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, apoptosis-regulated protein 1, apoptosis-regulated protein 2, or splicing factor Prp8.
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by the PRPF8 gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein (provided by RefSeq, Jul 2008). Mutations in the PRPF8 gene cause development of Retinitis pigmentosa 13 (RP13) characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors (van Lith-Verhoeven et al. 2002).
Rabbit anti Human PRPF8 antibody detects a band of 250 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by the PRPF8 gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein (provided by RefSeq, Jul 2008). Mutations in the PRPF8 gene cause development of Retinitis pigmentosa 13 (RP13) characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors (van Lith-Verhoeven et al. 2002).
Rabbit anti Human PRPF8 antibody detects a band of 250 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PRPF8 detects a band of approximately 250 kDa in K562 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human PRPF8
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q6P2Q9 Related reagents
- Entrez Gene
- PRPF8 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003723 RNA binding
- GO:0005681 spliceosomal complex
- GO:0005682 U5 snRNP
- GO:0007601 visual perception
- GO:0016607 nuclear speck
- GO:0050896 response to stimulus
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PRPF8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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