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PRPF19 antibody

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Western Blot Validated
Anti PRPF19 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRPF19 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 28 second exposure. Arrow points to PRPF19 (molecular weight 55 kDa)

Rabbit anti PRPF19

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00331
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human PRPF19 antibody recognizes PRPF19, also known as PRP19/PSO4 homolog, PRP19/PSO4 pre-mRNA processing factor 19 homolog, nuclear matrix protein 200, nuclear matrix protein NMP200 related to splicing factor PRP19, psoralen 4 and senescence evasion factor.

The PRPF19 gene is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al. 2008 PubMed 18263876, OMIM, Sep 2008).

Rabbit anti Human PRPF19 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PRPF19 detects a band of approximately 55 kDa in K562 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 4-33 of human PRPF19

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9UMS4
Entrez Gene
PRPF19
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0000209 protein polyubiquitination
GO:0000245 spliceosome assembly
GO:0003677 DNA binding
GO:0005681 spliceosomal complex
GO:0005819 spindle
GO:0006281 DNA repair
GO:0042802 identical protein binding
GO:0016607 nuclear speck
GO:0034450 ubiquitin-ubiquitin ligase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PRPF19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the PRPF19 specificity