Protein Kinase C zeta antibody

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Rabbit anti Human Protein Kinase C zeta (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1111 P* 50 µg
Rabbit anti human protein kinase c zeta polyclonal antibody recognizes PKCζ a serine/threonine kinase involved in a variety of cell processes including differentiation and proliferation. PKCζ activation is involved in the mitogen-activated protein kinase cascade, transcriptional factor NFkB activation, ribosomal S6-protein kinase signalling, and cell polarity.

In mice inhibition of expression of PHGDH by PKCζ acts at a tumor supressor and that loss of PKCζ allows tumor cells to proliferate under glucose deprived conditions leading to poor prognosis (Ma. et al.).

Rabbit anti human protein kinase c zeta detects an epitope within the N-terminal (NT) region of PKCζ. PKCζ is a member of the atypical PKC subfamily.
PKCζ may also be a potential therapeutic target in asthma, and a putative role in other pulmonary diseases, including chronic obstructive pulmonary disease (COPD).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to protein kinase C zeta were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide: near the N-terminus of human protein kinase C zeta.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q05513 Related reagents
Entrez Gene
PRKCZ Related reagents
GO Terms
GO:0004697 protein kinase C activity
GO:0005524 ATP binding
GO:0005768 endosome
GO:0006916 anti-apoptosis
GO:0008270 zinc ion binding
GO:0018105 peptidyl-serine phosphorylation
GO:0030168 platelet activation
GO:0031333 negative regulation of protein complex assembly
GO:0043560 insulin receptor substrate binding
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
Regulatory
For research purposes only

Applications of Protein Kinase C zeta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cerebellum.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Protein Kinase C zeta antibody

  1. Hirai, T. & Chida, K. (2003) Protein kinase Czeta (PKCzeta): activation mechanisms and cellular functions.
    J Biochem. 133 (1): 1-7.