Prohibitin antibody

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Rabbit anti Prohibitin

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00935
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human prohibitin antibody recognizes prohibitin, also known as PHB1.

PHB is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Anti-proliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of PHB have been identified. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013).

Rabbit anti Human prohibitin antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti prohibitin antibody recognizes a band of approximately 32 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human prohibitin

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P35232
Entrez Gene
PHB
GO Terms
GO:0006260 DNA replication
GO:0005887 integral to plasma membrane
GO:0005654 nucleoplasm
GO:0005743 mitochondrial inner membrane
GO:0008285 negative regulation of cell proliferation
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0042981 regulation of apoptosis
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0016575 histone deacetylation
GO:0042826 histone deacetylase binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0050847 progesterone receptor signaling pathway
GO:0060766 negative regulation of androgen receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Prohibitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml