PRO-IL-33 antibody

100% Secure


Rabbit anti Human Pro-Interleukin-33

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1626
Datasheet
IF WB
SDS
0.1 mg loader

Rabbit anti Human pro-interleukin-33 antibodyrecognises human pro-interleukin-33 (pro-IL-33). Pro-IL-33 is further processed into its mature secreted form, IL-33.

IL-33, a cytokine formerly known as NF-HEV or nuclear factor from high endothelieal venules, belongs to the IL-1 super family which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis.

IL-33 is abundantly expressed in high endothelial venules found in tonsils, peyers patches and mesenteric lymph nodes.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human pro-interleukin-33 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide sequence corresponding to an 19 amino acid sequence from near the amino terminus of Genbank accession No. NP_254274.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O95760
Entrez Gene
IL33
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0043032 positive regulation of macrophage activation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050729 positive regulation of inflammatory response
GO:0090197 positive regulation of chemokine secretion
Regulatory
For research purposes only

Applications of PRO-IL-33 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 20ug/ml
Western Blotting 1.0 2.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human brain tissue
Western Blotting
AHP1626 detects a band of approximately 36kDa in human lymph node cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml