PRMT1 antibody
Rabbit anti PRMT1
PRMT1 antibody recognizes protein arginine N-methyltransferase 1, also known as HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2 or interferon receptor 1-bound protein 4.
PRMT1 Antibody with Control Lysate
PRMT1 antibody recognizes protein arginine N-methyltransferase 1, also known as HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2 or interferon receptor 1-bound protein 4.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00236 | WB | 100 µl | ||||
| VPA00236KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PRMT1 antibody recognizes protein arginine N-methyltransferase 1, also known as HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2, histone-arginine N-methyltransferase and interferon receptor 1-bound protein 4.
Encoded by the PRMT1 gene, protein arginine N-methyltransferase 1 is a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of PRMT1 may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for PRMT1, and a pseudogene of PRMT1 is located on the long arm of chromosome 5 (provided by RefSeq, Dec 2011).
Rabbit anti Human PRMT1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the PRMT1 gene, protein arginine N-methyltransferase 1 is a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of PRMT1 may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for PRMT1, and a pseudogene of PRMT1 is located on the long arm of chromosome 5 (provided by RefSeq, Dec 2011).
Rabbit anti Human PRMT1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PRMT1 detects a band of approximately 42 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human PRMT1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q99873 Related reagents
- Entrez Gene
- PRMT1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PRMT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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