PRMT1 antibody

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Anti PRMT1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PRMT1 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to PRMT1 (molecular weight 42 kDa)

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Rabbit anti PRMT1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PRMT1

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Rabbit anti Human PRMT1 antibody recognizes protein arginine N-methyltransferase 1, also known as HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2, histone-arginine N-methyltransferase and interferon receptor 1-bound protein 4.

Encoded by the PRMT1 gene, protein arginine N-methyltransferase 1 is a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of PRMT1 may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for PRMT1, and a pseudogene of PRMT1 is located on the long arm of chromosome 5 (provided by RefSeq, Dec 2011).

Rabbit anti Human PRMT1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PRMT1 detects a band of approximately 42 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human PRMT1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.