PRESENILLIN-1 antibody

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Rabbit anti Presenilin-1

Rabbit anti human presenillin-1 antibody recognizes the presenillin-1 protein, also known as PS-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Presenilin-1 Antibody with Control Lysate

Rabbit anti human presenillin-1 antibody recognizes the presenillin-1 protein, also known as PS-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00332 WB 100 µl
VPA00332KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human presenilin-1 antibody recognizes presenilin-1 also known as PSEN1.

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (presenilin-1 or presenilin-2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for Presenilin-1, the full-length nature of only some have been determined (provided by RefSeq, Aug 2008).

Rabbit anti Human presenilin-1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti presenilin-1 detects a band of approximately 55 kDa in Raji cell lysate
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 330-359 of human presenilin-1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P49768 Related reagents
Entrez Gene
PSEN1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0000776 kinetochore
GO:0004175 endopeptidase activity
GO:0005887 integral to plasma membrane
GO:0005262 calcium channel activity
GO:0005640 nuclear outer membrane
GO:0005743 mitochondrial inner membrane
GO:0005765 lysosomal membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005790 smooth endoplasmic reticulum
GO:0005791 rough endoplasmic reticulum
GO:0005813 centrosome
GO:0005938 cell cortex
GO:0006509 membrane protein ectodomain proteolysis
GO:0006916 anti-apoptosis
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing
GO:0008013 beta-catenin binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0009986 cell surface
GO:0016324 apical plasma membrane
GO:0016337 cell-cell adhesion
GO:0030018 Z disc
GO:0030165 PDZ domain binding
GO:0030424 axon
GO:0030426 growth cone
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031594 neuromuscular junction
GO:0035253 ciliary rootlet
GO:0042987 amyloid precursor protein catabolic process
GO:0043025 neuronal cell body
GO:0043198 dendritic shaft
GO:0045121 membrane raft
GO:0045296 cadherin binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0050435 beta-amyloid metabolic process
GO:0051563 smooth endoplasmic reticulum calcium ion homeostasis
GO:0070765 gamma-secretase complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PRESENILLIN-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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